Induction of Multipotential Hematopoietic Progenitors from Human Pluripotent Stem Cells Via Respecification of Lineage-restricted Precursors

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2013 Oct 8

PMID 24094326

Citations 156

Authors

Sergei Doulatov

Linda T Vo

Stephanie S Chou

Peter G Kim

Natasha Arora

Hu Li

Brandon K Hadland

Irwin D Bernstein

James J Collins

Leonard I Zon

George Q Daley

Affiliations

Soon will be listed here.

Abstract

Human pluripotent stem cells (hPSCs) represent a promising source of patient-specific cells for disease modeling, drug screens, and cellular therapies. However, the inability to derive engraftable human hematopoietic stem and progenitor cells (HSPCs) has limited their characterization to in vitro assays. We report a strategy to respecify lineage-restricted CD34(+)CD45(+) myeloid precursors derived from hPSCs into multilineage progenitors that can be expanded in vitro and engrafted in vivo. HOXA9, ERG, and RORA conferred self-renewal and multilineage potential in vitro and maintained primitive CD34(+)CD38(-) cells. Screening cells via transplantation revealed that two additional factors, SOX4 and MYB, conferred engraftment. Progenitors specified with all five factors gave rise to reproducible short-term engraftment with myeloid and erythroid lineages. Erythroid precursors underwent hemoglobin switching in vivo, silencing embryonic and activating adult globin expression. Our combinatorial screening approach establishes a strategy for obtaining transcription-factor-mediated engraftment of blood progenitors from human pluripotent cells.

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