Patch and Whole-cell Voltage Clamp of Single Mammalian Visceral and Vascular Smooth Muscle Cells

Overview

Journal Experientia

Specialty Science

Date 1985 Jul 15

PMID 2408916

Citations 25

Authors

T B Bolton

R J Lang

T Takewaki

C D Benham

Affiliations

Soon will be listed here.

Abstract

Dispersal of the constituent cells of mammalian visceral and vascular smooth muscles has permitted recordings both of membrane currents under whole-cell voltage clamp, and of currents through single ionic channels using the patch-clamp technique. A rectangular depolarizing step applied to a single cell under voltage clamp yielded a net inward current followed by a net outward current in normal physiological solution. In isolated, 'inside-out' patches of cell membrane a calcium- and potential-sensitive K channel (100 pS conductance) and a calcium-insensitive, potential-sensitive K+ channel (50 pS conductance) with slow kinetics have so far been identified and characterized.

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