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Utility and Limitations of Spa-typing in Understanding the Epidemiology of Staphylococcus Aureus Bacteraemia Isolates in a Single University Hospital

Overview
Journal BMC Res Notes
Publisher Biomed Central
Specialties Biology
General Medicine
Date 2013 Oct 4
PMID 24088194
Citations 6
Authors
Giovanni Satta
Clare Louise Ling
Emma Shelley Cunningham
Timothy Daniel McHugh
Susan Hopkins
Affiliations
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Abstract

Background: Staphylococcus aureus (SA) is a recognized cause of nosocomial infections with 8,767 SA bacteraemia (SAB) cases reported in England only in 2012. Different typing methods have been developed but they are not generally performed as a routine investigation in hospital laboratories.

Findings: We collected epidemiological data and spa-typed all SAB isolates over a 12 months period. Spa-typing was useful to detect two potential outbreaks of methicillin-sensitive SA (MSSA). In addition, the analysis of spa-types from individuals with multiple bacteriaemias helped to distinguish between relapse and re-infection.

Conclusions: Spa-typing could be used as a rapid tool to understand the epidemiology of SAB, in particular the detection of hospital clusters and to distinguish relapse from re-infection, but clinicians should be aware of its possible limitations.

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References
1.
Vainio A, Koskela S, Virolainen A, Vuopio J, Salmenlinna S . Adapting spa typing for national laboratory-based surveillance of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 2011; 30(6):789-97. DOI: 10.1007/s10096-011-1158-5. View
2.
Lamagni T, Potz N, Powell D, Pebody R, Wilson J, Duckworth G . Mortality in patients with meticillin-resistant Staphylococcus aureus bacteraemia, England 2004-2005. J Hosp Infect. 2010; 77(1):16-20. DOI: 10.1016/j.jhin.2010.07.015. View
3.
Thwaites G . The management of Staphylococcus aureus bacteremia in the United Kingdom and Vietnam: a multi-centre evaluation. PLoS One. 2010; 5(12):e14170. PMC: 3001442. DOI: 10.1371/journal.pone.0014170. View
4.
Harris S, Cartwright E, Torok M, Holden M, Brown N, Ogilvy-Stuart A . Whole-genome sequencing for analysis of an outbreak of meticillin-resistant Staphylococcus aureus: a descriptive study. Lancet Infect Dis. 2012; 13(2):130-6. PMC: 3556525. DOI: 10.1016/S1473-3099(12)70268-2. View
5.
Sabat A, Budimir A, Nashev D, Sa-Leao R, van Dijl J, Laurent F . Overview of molecular typing methods for outbreak detection and epidemiological surveillance. Euro Surveill. 2013; 18(4):20380. DOI: 10.2807/ese.18.04.20380-en. View