Evaluation of the Oxidative Stress in Chronic Alcoholics

Overview

Journal J Clin Diagn Res

Specialty General Medicine

Date 2013 Oct 3

PMID 24086841

Citations 10

Authors

Mamta Singh

Seema Gupta

Udita Singhal

Rajesh Pandey

S K Aggarwal

Affiliations

Soon will be listed here.

Abstract

Aim: The present study was conducted to assess the activity of Gamma Glutamyl Transferase (GGT) and its association with oxidative stress in alcoholics.

Method: Sixty male alcoholics with a history of alcohol abuse for more than five years were the subjects of this study. Twenty healthy male volunteers who matched in age and the socio-economic status, served as the control subjects.

Results: GGT, reduced glutathione (GSH, a key intra-cellular antioxidant) and malondialdehyde (MDA, a marker of the oxidative stress) were assayed in the plasma of the two groups, and the results were statistically analyzed. The activity of the plasma GGT, known as a marker of Alcoholic Liver Disease (ALD); was significantly higher in the alcoholics as compared to that in the healthy controls.

Conclusion: There was a significant positive correlation between the enzyme activity and the plasma levels of MDA and this indicated that there was an increased release of this enzyme with enhanced oxidative damage, due to the generation of oxygen free radicals in the study group. There was a significantly increased level of MDA and a decrease in the level of GSH in the alcoholics as compared to those in the controls. Significant negative correlations between GGT and GSH, and between MDA and GSH were observed. The present study demonstrates that alcoholics have a compromised antioxidant defense system.

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References

Ishii H, Yasuraoka S, Shigeta Y, Takagi S, Kamiya T, Okuno F . Hepatic and intestinal gamma-glutamyltranspeptidase activity: its activation by chronic ethanol administration. Life Sci. 1978; 23(13):1393-7. DOI: 10.1016/0024-3205(78)90399-5. View

Tate S, Meister A . Subunit structure and isozymic forms of gamma-glutamyl transpeptidase. Proc Natl Acad Sci U S A. 1976; 73(8):2599-603. PMC: 430695. DOI: 10.1073/pnas.73.8.2599. View

Zhang H, Forman H, Choi J . Gamma-glutamyl transpeptidase in glutathione biosynthesis. Methods Enzymol. 2006; 401:468-83. DOI: 10.1016/S0076-6879(05)01028-1. View

Singh R, Ghosh S, Niaz M, Rastogi V, Wander G . Validation of tobacco and alcohol intake questionnaire in relation to food intakes for the Five City Study and a proposed classification for Indians. J Assoc Physicians India. 2002; 46(7):587-91. View

Pronko P, Bardina L, Satanovskaya V, Kuzmich A, Zimatkin S . Effect of chronic alcohol consumption on the ethanol- and acetaldehyde-metabolizing systems in the rat gastrointestinal tract. Alcohol Alcohol. 2002; 37(3):229-35. DOI: 10.1093/alcalc/37.3.229. View

Maher J . Exploring alcohol's effects on liver function. Alcohol Health Res World. 1997; 21(1):5-12. PMC: 6826796. View

Zhang X, Li S, Brown R, Ren J . Ethanol and acetaldehyde in alcoholic cardiomyopathy: from bad to ugly en route to oxidative stress. Alcohol. 2004; 32(3):175-86. DOI: 10.1016/j.alcohol.2004.01.005. View

Lieber C . Role of oxidative stress and antioxidant therapy in alcoholic and nonalcoholic liver diseases. Adv Pharmacol. 1997; 38:601-28. DOI: 10.1016/s1054-3589(08)61001-7. View

Beutler E, Duron O, Kelly B . Improved method for the determination of blood glutathione. J Lab Clin Med. 1963; 61:882-8. View

10.

Wlodek P, Sokolowska M, Smolenski O, Wlodek L . The gamma-glutamyltransferase activity and non-protein sulfhydryl compounds levels in rat kidney of different age groups. Acta Biochim Pol. 2002; 49(2):501-7. View

11.

Lieber C . Medical disorders of alcoholism. N Engl J Med. 1995; 333(16):1058-65. DOI: 10.1056/NEJM199510193331607. View

12.

Das S, Nayak P, Vasudevan D . Biochemical markers for alcohol consumption. Indian J Clin Biochem. 2012; 18(2):111-8. PMC: 3453888. DOI: 10.1007/BF02867376. View

13.

Ioannou G, Dominitz J, Weiss N, Heagerty P, Kowdley K . The effect of alcohol consumption on the prevalence of iron overload, iron deficiency, and iron deficiency anemia. Gastroenterology. 2004; 126(5):1293-301. DOI: 10.1053/j.gastro.2004.01.020. View

14.

Gupta S, Pandey R, Katyal R, Aggarwal H, Aggarwal R, Aggarwal S . Lipid peroxide levels and antioxidant status in alcoholic liver disease. Indian J Clin Biochem. 2012; 20(1):67-71. PMC: 3454176. DOI: 10.1007/BF02893045. View

15.

Whitehead T, Robinson D, Allaway S . The effects of cigarette smoking and alcohol consumption on serum liver enzyme activities: a dose-related study in men. Ann Clin Biochem. 1996; 33 ( Pt 6):530-5. DOI: 10.1177/000456329603300607. View

16.

Wu D, Cederbaum A . Alcohol, oxidative stress, and free radical damage. Alcohol Res Health. 2004; 27(4):277-84. PMC: 6668865. View

17.

Lee D, Blomhoff R, Jacobs Jr D . Is serum gamma glutamyltransferase a marker of oxidative stress?. Free Radic Res. 2004; 38(6):535-9. DOI: 10.1080/10715760410001694026. View

18.

Moriya S, Nagata S, Yokoyama H, Kato S, Horie Y, Ito T . Expression of gamma-glutamyl transpeptidase mRNA after depletion of glutathione in rat liver. Alcohol Alcohol Suppl. 1994; 29(1):107-11. View

19.

Nalpas B, Hispard E, Thepot V, Pot S, Dally S, Berthelot P . A comparative study between carbohydrate-deficient transferrin and gamma-glutamyltransferase for the diagnosis of excessive drinking in a liver unit. J Hepatol. 1998; 27(6):1003-8. DOI: 10.1016/s0168-8278(97)80143-2. View

20.

Kono S, Shinchi K, Imanishi K, TODOROKI I, Hatsuse K . Coffee and serum gamma-glutamyltransferase: a study of self-defense officials in Japan. Am J Epidemiol. 1994; 139(7):723-7. DOI: 10.1093/oxfordjournals.aje.a117062. View