Molecular Epidemiology of Clostridium Difficile Strains from Nosocomial-acquired Infections

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2013 Oct 2

PMID 24081935

Citations 2

Authors

Silvia Corbellini

Giorgio Piccinelli

Maria Antonia De Francesco

Giuseppe Ravizzola

Carlo Bonfanti

Affiliations

Soon will be listed here.

Abstract

The purpose of this study is to analyze isolates of Clostridium difficile from patients with nosocomial acquired infection in respect to their molecular type and antimicrobial susceptibility. Fifty-nine randomly selected clinical isolates were characterized. Molecular typing was performed by rep-PCR (DiversiLab). Isolates were tested by disk diffusion towards 11 different antibiotics. All isolates were susceptible to metronidazole and vancomycin. Fifty five (93 %) isolates were resistant to erythromycin and fifty six (95 %) exhibited resistance to both clindamycin and moxifloxacin. Twenty rep-PCR types were identified, but most clinical isolates formed four major rep-PCR clusters (A1 24/59, 40 %; A2 20/59, 33 %; A3 5/59, 8 %; A4 3/59, 5 %). These results show high genetic variability, which demonstrate clearly the complexity of the strains of C. difficile and also show an increasing rate of resistance to fluoroquinolones in our region emphasizing the importance of implementing surveillance programs in order to prevent further spread of resistance in C. difficile.

Citing Articles

Ribotyping of Clostridioides difficile in the Liberec Regional Hospital: a tertiary health care facility.

Kracik M, Dolinova I, Zemlickova H Folia Microbiol (Praha). 2022; 68(2):315-320.

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Clostridioides difficile Whole-genome Sequencing Differentiates Relapse With the Same Strain From Reinfection With a New Strain.

Cho J, Cunningham S, Pu M, Lennon R, Dens Higano J, Jeraldo P Clin Infect Dis. 2020; 72(5):806-813.

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