Sensitive Capture of Circulating Tumour Cells by Functionalized Graphene Oxide Nanosheets

Overview

Journal Nat Nanotechnol

Specialty Biotechnology

Date 2013 Oct 1

PMID 24077027

Citations 154

Authors

Hyeun Joong Yoon

Tae Hyun Kim

Zhuo Zhang

Ebrahim Azizi

Trinh M Pham

Costanza Paoletti

Jules Lin

Nithya Ramnath

Max S Wicha

Daniel F Hayes

Diane M Simeone

Sunitha Nagrath

Affiliations

Soon will be listed here.

Abstract

The spread of cancer throughout the body is driven by circulating tumour cells (CTCs). These cells detach from the primary tumour and move from the bloodstream to a new site of subsequent tumour growth. They also carry information about the primary tumour and have the potential to be valuable biomarkers for disease diagnosis and progression, and for the molecular characterization of certain biological properties of the tumour. However, the limited sensitivity and specificity of current methods for measuring and studying these cells in patient blood samples prevents the realization of their full clinical potential. The use of microfluidic devices is a promising method for isolating CTCs. However, the devices are reliant on three-dimensional structures, which limits further characterization and expansion of cells on the chip. Here we demonstrate an effective approach to isolating CTCs from blood samples of pancreatic, breast and lung cancer patients, by using functionalized graphene oxide nanosheets on a patterned gold surface. CTCs were captured with high sensitivity at a low concentration of target cells (73 ± 32.4% at 3-5 cells per ml blood).

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