Development of a Simple, Repeatable, and Cost-effective Extracellular Matrix for Long-term Xeno-free and Feeder-free Self-renewal of Human Pluripotent Stem Cells

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2013 Sep 26

PMID 24065274

Citations 5

Authors

Mohammad Pakzad

Mohammad Kazemi Ashtiani

Seyed Latif Mousavi-Gargari

Hossein Baharvand

Affiliations

Soon will be listed here.

Abstract

Given the potential importance of human pluripotent stem cells (hPSCs) in translational research and regenerative medicine, the aim of the present study was to develop a simple, safe, and cost-effective substrate to expand hPSCs. We report the development of an extracellular matrix (ECM), designated "RoGel," based on conditioned medium (CM) of human fibroblasts under serum- and xeno-free culture conditions. The long-term self-renewal of hPSCs on RoGel was also assessed. The results showed that self-renewal, pluripotency, plating efficiency, and cloning efficiency of hPSCs on this newly developed ECM were similar to those of Matrigel, the conventional mouse-cell line-derived ECM. The cells had the capability to passage mechanically on a cold surface, which resulted in their long-term maintenance with normal karyotype. We have demonstrated that CM-coated plates preserved for 1 year at room temperature maintained the capability of hPSC expansion. This ECM provides an attractive hPSC culture platform for both research and future therapeutic applications.

Citing Articles

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Ricci D, Nava M, Zandrini T, Cerullo G, Raimondi M, Osellame R Materials (Basel). 2017; 10(1).

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Two-photon polymerized "nichoid" substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions.

Nava M, Piuma A, Figliuzzi M, Cattaneo I, Bonandrini B, Zandrini T Stem Cell Res Ther. 2016; 7(1):132.

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The Histochemistry and Cell Biology pandect: the year 2014 in review.

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Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix.

Farzaneh Z, Pakzad M, Vosough M, Pournasr B, Baharvand H Histochem Cell Biol. 2014; 142(2):217-26.

PMID: 24477550 DOI: 10.1007/s00418-014-1183-4.

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