» Articles » PMID: 24065274

Development of a Simple, Repeatable, and Cost-effective Extracellular Matrix for Long-term Xeno-free and Feeder-free Self-renewal of Human Pluripotent Stem Cells

Overview
Publisher Springer
Date 2013 Sep 26
PMID 24065274
Citations 5
Authors
Affiliations
Soon will be listed here.
Abstract

Given the potential importance of human pluripotent stem cells (hPSCs) in translational research and regenerative medicine, the aim of the present study was to develop a simple, safe, and cost-effective substrate to expand hPSCs. We report the development of an extracellular matrix (ECM), designated "RoGel," based on conditioned medium (CM) of human fibroblasts under serum- and xeno-free culture conditions. The long-term self-renewal of hPSCs on RoGel was also assessed. The results showed that self-renewal, pluripotency, plating efficiency, and cloning efficiency of hPSCs on this newly developed ECM were similar to those of Matrigel, the conventional mouse-cell line-derived ECM. The cells had the capability to passage mechanically on a cold surface, which resulted in their long-term maintenance with normal karyotype. We have demonstrated that CM-coated plates preserved for 1 year at room temperature maintained the capability of hPSC expansion. This ECM provides an attractive hPSC culture platform for both research and future therapeutic applications.

Citing Articles

Scaling-Up Techniques for the Nanofabrication of Cell Culture Substrates via Two-Photon Polymerization for Industrial-Scale Expansion of Stem Cells.

Ricci D, Nava M, Zandrini T, Cerullo G, Raimondi M, Osellame R Materials (Basel). 2017; 10(1).

PMID: 28772424 PMC: 5344595. DOI: 10.3390/ma10010066.


Two-photon polymerized "nichoid" substrates maintain function of pluripotent stem cells when expanded under feeder-free conditions.

Nava M, Piuma A, Figliuzzi M, Cattaneo I, Bonandrini B, Zandrini T Stem Cell Res Ther. 2016; 7(1):132.

PMID: 27613598 PMC: 5016857. DOI: 10.1186/s13287-016-0387-z.


The Histochemistry and Cell Biology pandect: the year 2014 in review.

Taatjes D, Roth J Histochem Cell Biol. 2015; 143(4):339-68.

PMID: 25744491 DOI: 10.1007/s00418-015-1313-7.


The Histochem Cell Biol conspectus: the year 2013 in review.

Taatjes D, Roth J Histochem Cell Biol. 2014; 141(4):337-63.

PMID: 24610091 PMC: 7087837. DOI: 10.1007/s00418-014-1207-0.


Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix.

Farzaneh Z, Pakzad M, Vosough M, Pournasr B, Baharvand H Histochem Cell Biol. 2014; 142(2):217-26.

PMID: 24477550 DOI: 10.1007/s00418-014-1183-4.

References
1.
Irwin E, Gupta R, Dashti D, Healy K . Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells. Biomaterials. 2011; 32(29):6912-9. PMC: 3148342. DOI: 10.1016/j.biomaterials.2011.05.058. View

2.
Miyazaki T, Futaki S, Suemori H, Taniguchi Y, Yamada M, Kawasaki M . Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat Commun. 2012; 3:1236. PMC: 3535336. DOI: 10.1038/ncomms2231. View

3.
Carpenter M, Rosler E, Fisk G, Brandenberger R, Ares X, Miura T . Properties of four human embryonic stem cell lines maintained in a feeder-free culture system. Dev Dyn. 2004; 229(2):243-58. DOI: 10.1002/dvdy.10431. View

4.
Meng G, Liu S, Li X, Krawetz R, Rancourt D . Extracellular matrix isolated from foreskin fibroblasts supports long-term xeno-free human embryonic stem cell culture. Stem Cells Dev. 2009; 19(4):547-56. DOI: 10.1089/scd.2009.0303. View

5.
Steiner D, Khaner H, Cohen M, Even-Ram S, Gil Y, Itsykson P . Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol. 2010; 28(4):361-4. DOI: 10.1038/nbt.1616. View