The Clinical Utilization of Circulating Cell Free DNA (CCFDNA) in Blood of Cancer Patients

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2013 Sep 26

PMID 24065096

Citations 108

Authors

Yahya I Elshimali

Husseina Khaddour

Marianna Sarkissyan

Yanyuan Wu

Jaydutt V Vadgama

Affiliations

Soon will be listed here.

Abstract

Qualitative and quantitative testing of circulating cell free DNA (CCFDNA) can be applied for the management of malignant and benign neoplasms. Detecting circulating DNA in cancer patients may help develop a DNA profile for early stage diagnosis in malignancies. The technical issues of obtaining, using, and analyzing CCFDNA from blood will be discussed.

Citing Articles

Detection of Genomic Copy Number Variations in Ovarian Cancer in the Peripheral Blood System.

Wahl L, Hliabtsova U, Qian X, Klopf A, Hedemann N, Florkemeier I Cancers (Basel). 2025; 17(5).

PMID: 40075628 PMC: 11898772. DOI: 10.3390/cancers17050780.

Comparison of plasma and blood cell samples in metagenomic next-generation sequencing for identification of the causative pathogens of fever.

Wang D, Zhang Z, Shen H, Jin F, Liang J, Shen D Heliyon. 2024; 10(11):e31677.

PMID: 38841453 PMC: 11152940. DOI: 10.1016/j.heliyon.2024.e31677.

A comparative study on the detection of Mycobacterium leprae DNA in urine samples of leprosy patients using Rlep-PCR with other conventional samples.

David D, Das M, Mani Chandra H Mol Biol Rep. 2024; 51(1):504.

PMID: 38616219 DOI: 10.1007/s11033-024-09470-0.

Targeted Sequencing of Human Satellite 2 Repeat Sequences in Plasma cfDNA Reveals Potential Breast Cancer Biomarkers.

Gezer U, Oberhofer A, Worf K, Stoetzer O, Holdenrieder S, Bronkhorst A Diagnostics (Basel). 2024; 14(6).

PMID: 38535029 PMC: 10968943. DOI: 10.3390/diagnostics14060609.

Circulating Nucleic Acids in Colorectal Cancer: Diagnostic and Prognostic Value.

Igder S, Zamani M, Fakher S, Siri M, Ashktorab H, Azarpira N Dis Markers. 2024; 2024:9943412.

PMID: 38380073 PMC: 10878755. DOI: 10.1155/2024/9943412.

References

Bryzgunova O, Skvortsova T, Kolesnikova E, Starikov A, Rykova E, Vlassov V . Isolation and comparative study of cell-free nucleic acids from human urine. Ann N Y Acad Sci. 2006; 1075:334-40. DOI: 10.1196/annals.1368.045. View

Muller I, Beeger C, Alix-Panabieres C, Rebillard X, Pantel K, Schwarzenbach H . Identification of loss of heterozygosity on circulating free DNA in peripheral blood of prostate cancer patients: potential and technical improvements. Clin Chem. 2008; 54(4):688-96. DOI: 10.1373/clinchem.2007.099333. View

Jung K, Fleischhacker M, Rabien A . Cell-free DNA in the blood as a solid tumor biomarker--a critical appraisal of the literature. Clin Chim Acta. 2010; 411(21-22):1611-24. DOI: 10.1016/j.cca.2010.07.032. View

Nakayama G, Hibi K, Nakayama H, Kodera Y, Ito K, Akiyama S . A highly sensitive method for the detection of p16 methylation in the serum of colorectal cancer patients. Anticancer Res. 2007; 27(3B):1459-63. View

De Sario A . Clinical and molecular overview of inherited disorders resulting from epigenomic dysregulation. Eur J Med Genet. 2009; 52(6):363-72. DOI: 10.1016/j.ejmg.2009.07.004. View

Ng C, Zhang J, Wan S, Lee T, Arifi A, Mok T . Tumor p16M is a possible marker of advanced stage in non-small cell lung cancer. J Surg Oncol. 2002; 79(2):101-6. DOI: 10.1002/jso.10046. View

Agostini M, Enzo M, Bedin C, Belardinelli V, Goldin E, Del Bianco P . Circulating cell-free DNA: a promising marker of regional lymphonode metastasis in breast cancer patients. Cancer Biomark. 2012; 11(2-3):89-98. DOI: 10.3233/CBM-2012-0263. View

Van der Auwera I, Elst H, Van Laere S, Maes H, Huget P, van Dam P . The presence of circulating total DNA and methylated genes is associated with circulating tumour cells in blood from breast cancer patients. Br J Cancer. 2009; 100(8):1277-86. PMC: 2676551. DOI: 10.1038/sj.bjc.6605013. View

Beck J, Urnovitz H, Riggert J, Clerici M, Schutz E . Profile of the circulating DNA in apparently healthy individuals. Clin Chem. 2009; 55(4):730-8. DOI: 10.1373/clinchem.2008.113597. View

10.

Herbst A, Wallner M, Rahmig K, Stieber P, Crispin A, Lamerz R . Methylation of helicase-like transcription factor in serum of patients with colorectal cancer is an independent predictor of disease recurrence. Eur J Gastroenterol Hepatol. 2009; 21(5):565-9. DOI: 10.1097/MEG.0b013e328318ecf2. View

11.

Li J, Harris L, Mamon H, Kulke M, Liu W, Zhu P . Whole genome amplification of plasma-circulating DNA enables expanded screening for allelic imbalance in plasma. J Mol Diagn. 2006; 8(1):22-30. PMC: 1867563. DOI: 10.2353/jmoldx.2006.050074. View

12.

Muller H, Widschwendter A, Fiegl H, Ivarsson L, Goebel G, Perkmann E . DNA methylation in serum of breast cancer patients: an independent prognostic marker. Cancer Res. 2003; 63(22):7641-5. View

13.

Liggett T, Melnikov A, Marks J, Levenson V . Methylation patterns in cell-free plasma DNA reflect removal of the primary tumor and drug treatment of breast cancer patients. Int J Cancer. 2010; 128(2):492-9. PMC: 2970661. DOI: 10.1002/ijc.25363. View

14.

Sharma G, Mirza S, Parshad R, Gupta S, Ralhan R . DNA methylation of circulating DNA: a marker for monitoring efficacy of neoadjuvant chemotherapy in breast cancer patients. Tumour Biol. 2012; 33(6):1837-43. DOI: 10.1007/s13277-012-0443-y. View

15.

Zachariah R, Schmid S, Buerki N, Radpour R, Holzgreve W, Zhong X . Levels of circulating cell-free nuclear and mitochondrial DNA in benign and malignant ovarian tumors. Obstet Gynecol. 2008; 112(4):843-50. DOI: 10.1097/AOG.0b013e3181867bc0. View

16.

Bhayal A, Krishnaveni D, RangaRao K, Bogadi V, Suman C, Jyothy A . Role of tumor necrosis factor-α -308 G/A promoter polymorphism in gastric cancer. Saudi J Gastroenterol. 2013; 19(4):182-6. PMC: 3745661. DOI: 10.4103/1319-3767.114513. View

17.

Papadopoulou E, Davilas E, Sotiriou V, Georgakopoulos E, Georgakopoulou S, Koliopanos A . Cell-free DNA and RNA in plasma as a new molecular marker for prostate and breast cancer. Ann N Y Acad Sci. 2006; 1075:235-43. DOI: 10.1196/annals.1368.032. View

18.

Gahan P, Swaminathan R . Circulating nucleic acids in plasma and serum. Recent developments. Ann N Y Acad Sci. 2008; 1137:1-6. DOI: 10.1196/annals.1448.050. View

19.

Horvath A, Pakala S, Mudvari P, Reddy S, Ohshiro K, Casimiro S . Novel insights into breast cancer genetic variance through RNA sequencing. Sci Rep. 2013; 3:2256. PMC: 3722564. DOI: 10.1038/srep02256. View

20.

Lavon I, Refael M, Zelikovitch B, Shalom E, Siegal T . Serum DNA can define tumor-specific genetic and epigenetic markers in gliomas of various grades. Neuro Oncol. 2010; 12(2):173-80. PMC: 2940579. DOI: 10.1093/neuonc/nop041. View