Modulation of Lipid Metabolism by Deep-sea Water in Cultured Human Liver (HepG2) Cells

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2013 Sep 24

PMID 24057172

Citations 8

Authors

Shan He

Jiejie Hao

Weibing Peng

Peiju Qiu

Chunxia Li

Huashi Guan

Affiliations

Soon will be listed here.

Abstract

It has been found that deep-sea water was associated with lower serum lipid in animal model studies. Herein, we investigated whether DSW exerted a hypolipidemic activity and further elucidated how DSW modulated lipid metabolism in HepG2 cells. Preliminary animal studies showed that DSW exhibited potency to decrease serum total cholesterol, triglycerides, and LDL cholesterol, and increase HDL cholesterol, and the hepatic lipid contents were also significantly lower in the DSW group. When DSW was added to HepG2 cells, it decreased the lipid contents of hepatocyte through the activation of AMP-activated protein kinase, thus inhibiting the synthesis of cholesterol and fatty acid. Besides, LDL receptor was upregulated by activation of sterol regulatory element-binding protein-2. In addition, the levels of apolipoprotein AI and cholesterol 7-alpha-hydroxylase were also raised. Our investigation provided mechanisms by which DSW modulated lipid metabolism and indicated that DSW was worthy of further investigation and could be developed as functional drinking water in the prevention and treatment of hypolipidemic and other lifestyle-related diseases.

Citing Articles

Effect of Extract-Added Water Derived from Deep-Sea Water with Different Hardness on Cognitive Function, Motor Ability and Serum Indexes of Obese Mice.

Fukui K, Suzuki Y, Kato Y, Takeuchi N, Takenaka H, Kohno M Nutrients. 2022; 14(9).

PMID: 35565761 PMC: 9101427. DOI: 10.3390/nu14091794.

Deep Sea Water-Dissolved Organic Matter Intake Improves Hyperlipidemia and Inhibits Thrombus Formation and Vascular Inflammation in High-Fat Diet Hamsters.

Wu C, Cheng Y, Chen K, Chien C Life (Basel). 2022; 12(1).

PMID: 35054478 PMC: 8778340. DOI: 10.3390/life12010082.

Drinking Refined Deep-Sea Water Improves the Gut Ecosystem with Beneficial Effects on Intestinal Health in Humans: A Randomized Double-Blind Controlled Trial.

Takeuchi H, Higuchi K, Yoshikane Y, Takagi R, Tokuhiro S, Takenaka K Nutrients. 2020; 12(9).

PMID: 32878045 PMC: 7551512. DOI: 10.3390/nu12092646.

Combination Treatment of Deep Sea Water and Fucoidan Attenuates High Glucose-Induced Insulin-Resistance in HepG2 Hepatocytes.

He S, Peng W, Zhou H Mar Drugs. 2018; 16(2).

PMID: 29393871 PMC: 5852476. DOI: 10.3390/md16020048.

Potential Health Benefits of Deep Sea Water: A Review.

Mohd Nani S, Majid F, Jaafar A, Mahdzir A, Musa M Evid Based Complement Alternat Med. 2017; 2016:6520475.

PMID: 28105060 PMC: 5221345. DOI: 10.1155/2016/6520475.

References

Ditscheid B, Keller S, Jahreis G . Cholesterol metabolism is affected by calcium phosphate supplementation in humans. J Nutr. 2005; 135(7):1678-82. DOI: 10.1093/jn/135.7.1678. View

Hill S, McQueen M . Reverse cholesterol transport--a review of the process and its clinical implications. Clin Biochem. 1997; 30(7):517-25. DOI: 10.1016/s0009-9120(97)00098-2. View

Katsuda S, Yasukawa T, Nakagawa K, Miyake M, Yamasaki M, Katahira K . Deep-sea water improves cardiovascular hemodynamics in Kurosawa and Kusanagi-Hypercholesterolemic (KHC) rabbits. Biol Pharm Bull. 2008; 31(1):38-44. DOI: 10.1248/bpb.31.38. View

Ruderman N, Prentki M . AMP kinase and malonyl-CoA: targets for therapy of the metabolic syndrome. Nat Rev Drug Discov. 2004; 3(4):340-51. DOI: 10.1038/nrd1344. View

Fielding C, Fielding P . Intracellular cholesterol transport. J Lipid Res. 1997; 38(8):1503-21. View

Yoshioka S, Hamada A, Cui T, Yokota J, Yamamoto S, Kusunose M . Pharmacological activity of deep-sea water: examination of hyperlipemia prevention and medical treatment effect. Biol Pharm Bull. 2003; 26(11):1552-9. DOI: 10.1248/bpb.26.1552. View

Hwang H, Kim H, Lee S, Yun J . Anti-obesity and antidiabetic effects of deep sea water on ob/ob mice. Mar Biotechnol (NY). 2008; 11(4):531-9. DOI: 10.1007/s10126-008-9171-0. View

JAVITT N . Hep G2 cells as a resource for metabolic studies: lipoprotein, cholesterol, and bile acids. FASEB J. 1990; 4(2):161-8. DOI: 10.1096/fasebj.4.2.2153592. View

Shepherd J, Cobbe S, Ford I, Isles C, LORIMER A, Macfarlane P . Prevention of coronary heart disease with pravastatin in men with hypercholesterolemia. West of Scotland Coronary Prevention Study Group. N Engl J Med. 1995; 333(20):1301-7. DOI: 10.1056/NEJM199511163332001. View

10.

Marque S, Jacqmin-Gadda H, Dartigues J, Commenges D . Cardiovascular mortality and calcium and magnesium in drinking water: an ecological study in elderly people. Eur J Epidemiol. 2003; 18(4):305-9. DOI: 10.1023/a:1023618728056. View

11.

Jacqmain M, Doucet E, Despres J, Bouchard C, Tremblay A . Calcium intake, body composition, and lipoprotein-lipid concentrations in adults. Am J Clin Nutr. 2003; 77(6):1448-52. DOI: 10.1093/ajcn/77.6.1448. View

12.

Rayssiguier Y . Magnesium, lipids and vascular diseases. Experimental evidence in animal models. Magnesium. 1986; 5(3-4):182-90. View

13.

He K, Liu K, Daviglus M, Morris S, Loria C, Van Horn L . Magnesium intake and incidence of metabolic syndrome among young adults. Circulation. 2006; 113(13):1675-82. DOI: 10.1161/CIRCULATIONAHA.105.588327. View

14.

Gerber R, Ryan J, Clark D . Cell-based screen of HMG-CoA reductase inhibitors and expression regulators using LC-MS. Anal Biochem. 2004; 329(1):28-34. DOI: 10.1016/j.ab.2004.03.023. View

15.

Cefalu W, Wang Z, Zhang X, Baldor L, Russell J . Oral chromium picolinate improves carbohydrate and lipid metabolism and enhances skeletal muscle Glut-4 translocation in obese, hyperinsulinemic (JCR-LA corpulent) rats. J Nutr. 2002; 132(6):1107-14. DOI: 10.1093/jn/132.6.1107. View

16.

Hwang H, Kim S, Yoo Y, Chu Y, Shon Y, Nam K . Inhibitory effect of deep-sea water on differentiation of 3T3-L1 adipocytes. Mar Biotechnol (NY). 2008; 11(2):161-8. DOI: 10.1007/s10126-008-9131-8. View

17.

Winder W, Hardie D . AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes. Am J Physiol. 1999; 277(1):E1-10. DOI: 10.1152/ajpendo.1999.277.1.E1. View

18.

Dueland S, Drisko J, Graf L, Machleder D, Lusis A, Davis R . Effect of dietary cholesterol and taurocholate on cholesterol 7 alpha-hydroxylase and hepatic LDL receptors in inbred mice. J Lipid Res. 1993; 34(6):923-31. View

19.

Ravn H, Korsholm T, Falk E . Oral magnesium supplementation induces favorable antiatherogenic changes in ApoE-deficient mice. Arterioscler Thromb Vasc Biol. 2001; 21(5):858-62. DOI: 10.1161/01.atv.21.5.858. View

20.

Tsuchiya Y, Watanabe A, Fujisawa N, Kaneko T, Ishizu T, Fujimoto T . Effects of desalted deep seawater on hematologic and blood chemical values in mice. Tohoku J Exp Med. 2004; 203(3):175-82. DOI: 10.1620/tjem.203.175. View