Induced Pluripotency for Translational Research

Overview

Journal Genomics Proteomics Bioinformatics

Specialty Biology

Date 2013 Sep 24

PMID 24056061

Citations 4

Authors

Menghua Wu

Guilai Chen

Baoyang Hu

Affiliations

Soon will be listed here.

Abstract

The advent of induced pluripotent stem cells (iPSCs) has revolutionized the concept of cellular reprogramming and potentially will solve the immunological compatibility issues that have so far hindered the application of human pluripotent stem cells in regenerative medicine. Recent findings showed that pluripotency is defined by a state of balanced lineage potency, which can be artificially instated through various procedures, including the conventional Yamanaka strategy. As a type of pluripotent stem cell, iPSCs are subject to the usual concerns over purity of differentiated derivatives and risks of tumor formation when used for cell-based therapy, though they provide certain advantages in translational research, especially in the areas of personalized medicine, disease modeling and drug screening. iPSC-based technology, human embryonic stem cells (hESCs) and direct lineage conversion each will play distinct roles in specific aspects of translational medicine, and continue yielding surprises for scientists and the public.

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References

Chae J, Kim D, Lee N, Jeon Y, Jeon I, Kwon J . Quantitative proteomic analysis of induced pluripotent stem cells derived from a human Huntington's disease patient. Biochem J. 2012; 446(3):359-71. DOI: 10.1042/BJ20111495. View

Hanna J, Wernig M, Markoulaki S, Sun C, Meissner A, Cassady J . Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science. 2007; 318(5858):1920-3. DOI: 10.1126/science.1152092. View

Hu B, Zhang S . Differentiation of spinal motor neurons from pluripotent human stem cells. Nat Protoc. 2009; 4(9):1295-304. PMC: 2789120. DOI: 10.1038/nprot.2009.127. View

Kondo T, Asai M, Tsukita K, Kutoku Y, Ohsawa Y, Sunada Y . Modeling Alzheimer's disease with iPSCs reveals stress phenotypes associated with intracellular Aβ and differential drug responsiveness. Cell Stem Cell. 2013; 12(4):487-96. DOI: 10.1016/j.stem.2013.01.009. View

Briggs R, King T . Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs. Proc Natl Acad Sci U S A. 1952; 38(5):455-63. PMC: 1063586. DOI: 10.1073/pnas.38.5.455. View

Wilmut I, Schnieke A, McWhir J, Kind A, Campbell K . Viable offspring derived from fetal and adult mammalian cells. Nature. 1997; 385(6619):810-3. DOI: 10.1038/385810a0. View

Shu J, Wu C, Wu Y, Li Z, Shao S, Zhao W . Induction of pluripotency in mouse somatic cells with lineage specifiers. Cell. 2013; 153(5):963-75. PMC: 4640445. DOI: 10.1016/j.cell.2013.05.001. View

Hou P, Li Y, Zhang X, Liu C, Guan J, Li H . Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science. 2013; 341(6146):651-4. DOI: 10.1126/science.1239278. View

Gurdon J, Laskey R, Reeves O . The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. J Embryol Exp Morphol. 1975; 34(1):93-112. View

10.

Xie H, Ye M, Feng R, Graf T . Stepwise reprogramming of B cells into macrophages. Cell. 2004; 117(5):663-76. DOI: 10.1016/s0092-8674(04)00419-2. View

11.

Marchetto M, Carromeu C, Acab A, Yu D, Yeo G, Mu Y . A model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells. Cell. 2010; 143(4):527-39. PMC: 3003590. DOI: 10.1016/j.cell.2010.10.016. View

12.

Brennand K, Simone A, Jou J, Gelboin-Burkhart C, Tran N, Sangar S . Modelling schizophrenia using human induced pluripotent stem cells. Nature. 2011; 473(7346):221-5. PMC: 3392969. DOI: 10.1038/nature09915. View

13.

Zhao X, Li W, Lv Z, Liu L, Tong M, Hai T . iPS cells produce viable mice through tetraploid complementation. Nature. 2009; 461(7260):86-90. DOI: 10.1038/nature08267. View

14.

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

15.

Li R, Liang J, Ni S, Zhou T, Qing X, Li H . A mesenchymal-to-epithelial transition initiates and is required for the nuclear reprogramming of mouse fibroblasts. Cell Stem Cell. 2010; 7(1):51-63. DOI: 10.1016/j.stem.2010.04.014. View

16.

Schneuwly S, Klemenz R, Gehring W . Redesigning the body plan of Drosophila by ectopic expression of the homoeotic gene Antennapedia. Nature. 1987; 325(6107):816-8. DOI: 10.1038/325816a0. View

17.

Kim J, Hoffman J, Alpaugh R, Rhim A, Rhimm A, Reichert M . An iPSC line from human pancreatic ductal adenocarcinoma undergoes early to invasive stages of pancreatic cancer progression. Cell Rep. 2013; 3(6):2088-99. PMC: 3726210. DOI: 10.1016/j.celrep.2013.05.036. View

18.

Park I, Zhao R, West J, Yabuuchi A, Huo H, Ince T . Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2007; 451(7175):141-6. DOI: 10.1038/nature06534. View

19.

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

20.

Samavarchi-Tehrani P, Golipour A, David L, Sung H, Beyer T, Datti A . Functional genomics reveals a BMP-driven mesenchymal-to-epithelial transition in the initiation of somatic cell reprogramming. Cell Stem Cell. 2010; 7(1):64-77. DOI: 10.1016/j.stem.2010.04.015. View