Cell Type-specific Post-Golgi Apparatus Localization of a "resident" Endoplasmic Reticulum Glycoprotein, Glucosidase II

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1990 Feb 1

PMID 2404989

Citations 9

Authors

D BRADA

D Kerjaschki

J Roth

Affiliations

Soon will be listed here.

Abstract

Glucosidase II, an asparagine-linked oligosaccharide processing enzyme, is a resident glycoprotein of the endoplasmic reticulum. In kidney tubular cells, in contrast to previous findings on hepatocytes, we found by light and electron microscopy immunoreactivity for glucosidase II predominantly in post-Golgi apparatus structures. The majority of immunolabel was in endocytotic structures beneath the plasma membrane. Immunoprecipitation confirmed presence of the glucosidase II subunit in purified brush border preparations. Kidney glucosidase II contained species carrying endo H-sensitive, high mannose as well as endo H-resistant oligosaccharide chains. Some species of glucosidase II contained sialic acid. The sialylated species were enzymatically active. This study demonstrates than an enzyme presumed to be a resident of the endoplasmic reticulum may show alternative localizations in some cell types.

Citing Articles

Expression of α-subunit of α-glucosidase II in adult mouse brain regions and selected organs.

Anji A, Miller H, Raman C, Phillips M, Ciment G, Kumari M J Neurosci Res. 2014; 93(1):82-93.

PMID: 25131991 PMC: 4237662. DOI: 10.1002/jnr.23470.

Proteomic profiling of phosphoproteins and glycoproteins responsive to wild-type alpha-synuclein accumulation and aggregation.

Kulathingal J, Ko L, Cusack B, Yen S Biochim Biophys Acta. 2008; 1794(2):211-24.

PMID: 19027885 PMC: 2679264. DOI: 10.1016/j.bbapap.2008.09.025.

Cysts of PRKCSH mutated polycystic liver disease patients lack hepatocystin but express Sec63p.

Waanders E, Croes H, Maass C, Te Morsche R, van Geffen H, van Krieken J Histochem Cell Biol. 2008; 129(3):301-10.

PMID: 18224332 DOI: 10.1007/s00418-008-0381-3.

Cloning and characterization of the glucosidase II alpha subunit gene of Trichoderma reesei: a frameshift mutation results in the aberrant glycosylation profile of the hypercellulolytic strain Rut-C30.

Geysens S, Pakula T, Uusitalo J, Dewerte I, Penttila M, Contreras R Appl Environ Microbiol. 2005; 71(6):2910-24.

PMID: 15932985 PMC: 1151825. DOI: 10.1128/AEM.71.6.2910-2924.2005.

Immunolocalization of vacuolar system-associated protein-60 (VASAP-60).

Brule S, Faure R, Dore M, Silversides D, Lussier J Histochem Cell Biol. 2003; 119(5):371-81.

PMID: 12750905 DOI: 10.1007/s00418-003-0521-8.

References

Galteau M, Antoine B, Reggio H . Epoxide hydrolase is a marker for the smooth endoplasmic reticulum in rat liver. EMBO J. 1985; 4(11):2793-800. PMC: 554580. DOI: 10.1002/j.1460-2075.1985.tb04005.x. View

Poruchynsky M, Tyndall C, Both G, Sato F, Bellamy A, Atkinson P . Deletions into an NH2-terminal hydrophobic domain result in secretion of rotavirus VP7, a resident endoplasmic reticulum membrane glycoprotein. J Cell Biol. 1985; 101(6):2199-209. PMC: 2113985. DOI: 10.1083/jcb.101.6.2199. View

Manigley C, Roth J . Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique. J Histochem Cytochem. 1985; 33(12):1247-51. DOI: 10.1177/33.12.2415576. View

Roth J, Taatjes D, Lucocq J, Weinstein J, Paulson J . Demonstration of an extensive trans-tubular network continuous with the Golgi apparatus stack that may function in glycosylation. Cell. 1985; 43(1):287-95. DOI: 10.1016/0092-8674(85)90034-0. View

RODMAN J, Seidman L, Farquhar M . The membrane composition of coated pits, microvilli, endosomes, and lysosomes is distinctive in the rat kidney proximal tubule cell. J Cell Biol. 1986; 102(1):77-87. PMC: 2114052. DOI: 10.1083/jcb.102.1.77. View

Lucocq J, BRADA D, Roth J . Immunolocalization of the oligosaccharide trimming enzyme glucosidase II. J Cell Biol. 1986; 102(6):2137-46. PMC: 2114248. DOI: 10.1083/jcb.102.6.2137. View

Kreis T, Lodish H . Oligomerization is essential for transport of vesicular stomatitis viral glycoprotein to the cell surface. Cell. 1986; 46(6):929-37. PMC: 7133264. DOI: 10.1016/0092-8674(86)90075-9. View

Gething M, McCammon K, Sambrook J . Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport. Cell. 1986; 46(6):939-50. DOI: 10.1016/0092-8674(86)90076-0. View

Roth J, Taatjes D, Weinstein J, Paulson J, Greenwell P, WATKINS W . Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cells. J Biol Chem. 1986; 261(30):14307-12. View

10.

Copeland C, Doms R, Bolzau E, Webster R, Helenius A . Assembly of influenza hemagglutinin trimers and its role in intracellular transport. J Cell Biol. 1986; 103(4):1179-91. PMC: 2114319. DOI: 10.1083/jcb.103.4.1179. View

11.

Munro S, Pelham H . A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987; 48(5):899-907. DOI: 10.1016/0092-8674(87)90086-9. View

12.

Kaushal G, Pastuszak I, Hatanaka K, Elbein A . Plant glucosidase II catalyzes a transglucosylation reaction in addition to the hydrolytic reaction. Arch Biochem Biophys. 1989; 272(2):481-7. DOI: 10.1016/0003-9861(89)90243-9. View

13.

Paabo S, Bhat B, Wold W, Peterson P . A short sequence in the COOH-terminus makes an adenovirus membrane glycoprotein a resident of the endoplasmic reticulum. Cell. 1987; 50(2):311-7. PMC: 7133293. DOI: 10.1016/0092-8674(87)90226-1. View

14.

Rothman J . Protein sorting by selective retention in the endoplasmic reticulum and Golgi stack. Cell. 1987; 50(4):521-2. PMC: 7133322. DOI: 10.1016/0092-8674(87)90024-9. View

15.

Hirschberg C, Snider M . Topography of glycosylation in the rough endoplasmic reticulum and Golgi apparatus. Annu Rev Biochem. 1987; 56:63-87. DOI: 10.1146/annurev.bi.56.070187.000431. View

16.

Brown J, NOVAK E, Takeuchi K, Moore K, Medda S, Swank R . Lumenal location of the microsomal beta-glucuronidase-egasyn complex. J Cell Biol. 1987; 105(4):1571-8. PMC: 2114662. DOI: 10.1083/jcb.105.4.1571. View

17.

Lodish H . Transport of secretory and membrane glycoproteins from the rough endoplasmic reticulum to the Golgi. A rate-limiting step in protein maturation and secretion. J Biol Chem. 1988; 263(5):2107-10. View

18.

Taatjes D, Roth J, Weinstein J, Paulson J . Post-Golgi apparatus localization and regional expression of rat intestinal sialyltransferase detected by immunoelectron microscopy with polypeptide epitope-purified antibody. J Biol Chem. 1988; 263(13):6302-9. View

19.

Ceriotti A, Colman A . Binding to membrane proteins within the endoplasmic reticulum cannot explain the retention of the glucose-regulated protein GRP78 in Xenopus oocytes. EMBO J. 1988; 7(3):633-8. PMC: 454367. DOI: 10.1002/j.1460-2075.1988.tb02857.x. View

20.

Pelham H . Evidence that luminal ER proteins are sorted from secreted proteins in a post-ER compartment. EMBO J. 1988; 7(4):913-8. PMC: 454416. DOI: 10.1002/j.1460-2075.1988.tb02896.x. View