A Simplified Positive-sense-RNA Virus Construction Approach That Enhances Analysis Throughput

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2013 Sep 20

PMID 24049164

Citations 36

Authors

Bunpote Siridechadilok

Methee Gomutsukhavadee

Thunyarat Sawaengpol

Sutha Sangiambut

Chunya Puttikhunt

Kwanrutai Chin-Inmanu

Prapat Suriyaphol

Prida Malasit

Gavin Screaton

Juthathip Mongkolsapaya

Affiliations

Soon will be listed here.

Abstract

Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.

Citing Articles

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