Production of Monoclonal Antibody Against ORF72 of Koi Herpesvirus Isolated in Taiwan

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2013 Sep 19

PMID 24046233

Citations 3

Authors

Chien Tu

Yi-Ping Lu

Chia-Yu Hsieh

Su-Ming Huang

Shao-Kuang Chang

Meei-Mei Chen

Affiliations

Soon will be listed here.

Abstract

A monoclonal antibody (MAb) was generated against the capsid protein (ORF 72) of koi herpesvirus (KHV) isolated from diseased koi Cyprinus carpio in Taiwan. The clone of MAb-B2 was obtained by immunizing mice with whole virus particles and further identified using indirect enzyme-linked immunosorbent assay and Western blot assay. In addition, it detected KHV in KHV-infected cells but not in those of mock-infected cells as demonstrated by indirect immunofluorescence assay. The neutralization test showed that MAb-B2 neutralized KHV. Furthermore, we uncovered that MAb-B2 recognizes the ORF72 of KHV as revealed by liquid chromatography-tandem mass spectrometry and Western blot assays. Additionally, MAb-B2 has been used as a diagnostic tool for detection of KHV in clinical samples by immunohistochemistry. Collectively, our results indicated that MAb-B2 could be used in the development of a diagnostic kit for diagnosis of KHV infections and ORF72 protein of KHV might be a candidate for future vaccine development.

Citing Articles

Inoculation of cyprinid herpesvirus 3 (CyHV-3) on common carp brain cells-influence of process parameters on virus yield.

Mletzko A, Amtmann A, Bergmann S, Lee P, Christian J, Buchholz R In Vitro Cell Dev Biol Anim. 2017; 53(7):579-585.

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Rapid Detection of Cyprinid Herpesvirus 3 in Latently Infected Koi by Recombinase Polymerase Amplification.

Prescott M, Reed A, Jin L, Pastey M J Aquat Anim Health. 2016; 28(3):173-80.

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Expression of immunogenic structural proteins of cyprinid herpesvirus 3 in vitro assessed using immunofluorescence.

Monaghan S, Thompson K, Bron J, Bergmann S, Jung T, Aoki T Vet Res. 2016; 47:8.

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