The Long Coiled-coil Protein NECC2 is Associated to Caveolae and Modulates NGF/TrkA Signaling in PC12 Cells [corrected]

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Sep 17

PMID 24040018

Citations 5

Authors

Alberto Diaz-Ruiz

Yoana Rabanal-Ruiz

Andres Travez

Francisco Gracia-Navarro

David Cruz-Garcia

Maite Montero-Hadjadje

Youssef Anouar

Stephane Gasman

Nicolas Vitale

Rafael Vazquez-Martinez

Maria M Malagon

Affiliations

Soon will be listed here.

Abstract

TrkA-mediated NGF signaling in PC12 cells has been shown to be compartimentalized in specialized microdomains of the plasma membrane, the caveolae, which are organized by scaffold proteins including the member of the caveolin family of proteins, caveolin-1. Here, we characterize the intracellular distribution as well as the biochemical and functional properties of the neuroendocrine long coiled-coil protein 2 (NECC2), a novel long coiled-coil protein selectively expressed in neuroendocrine tissues that contains a predicted caveolin-binding domain and displays structural characteristics of a scaffolding factor. NECC2 distributes in caveolae, wherein it colocalizes with the TrkA receptor, and behaves as a caveolae-associated protein in neuroendocrine PC12 cells. In addition, stimulation of PC12 cells with nerve growth factor (NGF) increased the expression and regulated the distribution of NECC2. Interestingly, knockdown as well as overexpression of NECC2 resulted in a reduction of NGF-induced phosphorylation of the TrkA downstream effector extracellular signal-regulated kinases 1 and 2 (ERK1/ERK2) but not of Akt. Altogether, our results identify NECC2 as a novel component of caveolae in PC12 cells and support the contribution of this protein in the maintenance of TrkA-mediated NGF signaling.

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