Transcription of the Promoter of the Rat NF-1 Gene Depends on the Integrity of an Sp1 Recognition Site

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1990 Jan 1

PMID 2403642

Citations 6

Authors

R Ammendola

F Gounari

G Piaggio

V De Simone

R Cortese

Affiliations

Soon will be listed here.

Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

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