Molecular Architecture of the ATP-dependent Chromatin-remodeling Complex SWR1

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2013 Sep 17

PMID 24034246

Citations 79

Authors

Vu Q Nguyen

Anand Ranjan

Florian Stengel

Debbie Wei

Ruedi Aebersold

Carl Wu

Andres E Leschziner

Affiliations

Soon will be listed here.

Abstract

The ATP-dependent chromatin-remodeling complex SWR1 exchanges a variant histone H2A.Z/H2B dimer for a canonical H2A/H2B dimer at nucleosomes flanking histone-depleted regions, such as promoters. This localization of H2A.Z is conserved throughout eukaryotes. SWR1 is a 1 megadalton complex containing 14 different polypeptides, including the AAA+ ATPases Rvb1 and Rvb2. Using electron microscopy, we obtained the three-dimensional structure of SWR1 and mapped its major functional components. Our data show that SWR1 contains a single heterohexameric Rvb1/Rvb2 ring that, together with the catalytic subunit Swr1, brackets two independently assembled multisubunit modules. We also show that SWR1 undergoes a large conformational change upon engaging a limited region of the nucleosome core particle. Our work suggests an important structural role for the Rvbs and a distinct substrate-handling mode by SWR1, thereby providing a structural framework for understanding the complex dimer-exchange reaction.

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