Optimization of Nutrition Factors on Chitinase Production from a Newly Isolated Chitiolyticbacter Meiyuanensis SYBC-H1

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2013 Sep 14

PMID 24031816

Citations 7

Authors

Zhikui Hao

Yujie Cai

Xiangru Liao

Xiaoli Zhang

Zhiyou Fang

Dabing Zhang

Affiliations

Soon will be listed here.

Abstract

The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.

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