Prevalence of Extended-spectrum Beta-lactamases-producing Microorganisms in Nosocomial Patients and Molecular Characterization of the Shv Type Isolates

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2013 Sep 14

PMID 24031491

Citations 8

Authors

Caio Fernando de Oliveira

Adenilde Salla

Valeria Maria Lara

Alexandre Rieger

Jorge Andre Horta

Sydney Hartz Alves

Affiliations

Soon will be listed here.

Abstract

The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.

Citing Articles

Extended-Spectrum Beta-Lactamases Producing in South America: A Systematic Review with a One Health Perspective.

Bastidas-Caldes C, Romero-Alvarez D, Valdez-Velez V, Morales R, Montalvo-Hernandez A, Gomes-Dias C Infect Drug Resist. 2022; 15:5759-5779.

PMID: 36204394 PMC: 9531622. DOI: 10.2147/IDR.S371845.

Multidrug-resistant bacteria with ESBL genes: a growing threat among people living with HIV/AIDS in Nepal.

Maharjan R, Bastola A, Adhikari N, Rijal K, Banjara M, Ghimire P BMC Infect Dis. 2022; 22(1):526.

PMID: 35672713 PMC: 9171981. DOI: 10.1186/s12879-022-07503-2.

Resistance Patterns and Phenotypic Detection of β-lactamase Enzymes among Enterobacteriaceae Isolates from Referral Hospitals in Khartoum State, Sudan.

Dirar M, Bilal N, Ibrahim M, Hamid M Cureus. 2020; 12(3):e7260.

PMID: 32195070 PMC: 7075475. DOI: 10.7759/cureus.7260.

Molecular characterization of extended-spectrum β-lactamases among clinical isolates of & : A multi-centric study from tertiary care hospitals in India.

Gautam V, Thakur A, Sharma M, Singh A, Bansal S, Sharma A Indian J Med Res. 2019; 149(2):208-215.

PMID: 31219085 PMC: 6563744. DOI: 10.4103/ijmr.IJMR_172_18.

Molecular Characterization of Extended-Spectrum β-Lactamase Isolated from Egyptian Patients with Community- and Hospital-Acquired Urinary Tract Infection.

Shash R, Elshimy A, Soliman M, Mosharafa A Am J Trop Med Hyg. 2018; 100(3):522-528.

PMID: 30594263 PMC: 6402938. DOI: 10.4269/ajtmh.18-0396.

References

Jeong S, Bae I, Lee J, Sohn S, Kang G, Jeon G . Molecular characterization of extended-spectrum beta-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli from a Korean nationwide survey. J Clin Microbiol. 2004; 42(7):2902-6. PMC: 446292. DOI: 10.1128/JCM.42.7.2902-2906.2004. View

Coque T, Oliver A, Baquero F, Canton R . Genes encoding TEM-4, SHV-2, and CTX-M-10 extended-spectrum beta-lactamases are carried by multiple Klebsiella pneumoniae clones in a single hospital (Madrid, 1989 to 2000). Antimicrob Agents Chemother. 2002; 46(2):500-10. PMC: 127031. DOI: 10.1128/AAC.46.2.500-510.2002. View

Paterson D . Recommendation for treatment of severe infections caused by Enterobacteriaceae producing extended-spectrum beta-lactamases (ESBLs). Clin Microbiol Infect. 2001; 6(9):460-3. DOI: 10.1046/j.1469-0691.2000.00107.x. View

Sanguinetti M, Posteraro B, Spanu T, Ciccaglione D, Romano L, Fiori B . Characterization of clinical isolates of Enterobacteriaceae from Italy by the BD Phoenix extended-spectrum beta-lactamase detection method. J Clin Microbiol. 2003; 41(4):1463-8. PMC: 153891. DOI: 10.1128/JCM.41.4.1463-1468.2003. View

Tofteland S, Haldorsen B, Dahl K, Simonsen G, Steinbakk M, Walsh T . Effects of phenotype and genotype on methods for detection of extended-spectrum-beta-lactamase-producing clinical isolates of Escherichia coli and Klebsiella pneumoniae in Norway. J Clin Microbiol. 2006; 45(1):199-205. PMC: 1828980. DOI: 10.1128/JCM.01319-06. View

Luzzaro F, Mezzatesta M, Mugnaioli C, Perilli M, Stefani S, Amicosante G . Trends in production of extended-spectrum beta-lactamases among enterobacteria of medical interest: report of the second Italian nationwide survey. J Clin Microbiol. 2006; 44(5):1659-64. PMC: 1479207. DOI: 10.1128/JCM.44.5.1659-1664.2006. View

Paterson D, Bonomo R . Extended-spectrum beta-lactamases: a clinical update. Clin Microbiol Rev. 2005; 18(4):657-86. PMC: 1265908. DOI: 10.1128/CMR.18.4.657-686.2005. View

Perilli M, DellAmico E, Segatore B, De Massis M, Bianchi C, Luzzaro F . Molecular characterization of extended-spectrum beta-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey. J Clin Microbiol. 2002; 40(2):611-4. PMC: 153390. DOI: 10.1128/JCM.40.2.611-614.2002. View

dAzevedo P, Goncalves A, Musskopf M, Ramos C, Dias C . Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing. Braz J Infect Dis. 2005; 8(5):372-7. DOI: 10.1590/s1413-86702004000500006. View

10.

Steward C, Rasheed J, Hubert S, Biddle J, Raney P, Anderson G . Characterization of clinical isolates of Klebsiella pneumoniae from 19 laboratories using the National Committee for Clinical Laboratory Standards extended-spectrum beta-lactamase detection methods. J Clin Microbiol. 2001; 39(8):2864-72. PMC: 88252. DOI: 10.1128/JCM.39.8.2864-2872.2001. View

11.

Carter M, Oakton K, Warner M, Livermore D . Detection of extended-spectrum beta-lactamases in klebsiellae with the Oxoid combination disk method. J Clin Microbiol. 2000; 38(11):4228-32. PMC: 87569. DOI: 10.1128/JCM.38.11.4228-4232.2000. View

12.

van Soolingen D, Hermans P, de Haas P, Soll D, van Embden J . Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis. J Clin Microbiol. 1991; 29(11):2578-86. PMC: 270376. DOI: 10.1128/jcm.29.11.2578-2586.1991. View