Nested Polymerase Chain Reaction and Sequence- Based Detection of Leishmania Infection of Sand Flies in Recently Emerged Endemic Focus of Zoonotic Cutaneous Leishmaniasis, Southern Iran

Overview

Journal Iran J Med Sci

Publisher Shiraz University of Medical Sciences

Specialty General Medicine

Date 2013 Sep 14

PMID 24031105

Citations 8

Authors

Kourosh Azizi

Abdollah Badzohreh

Bahador Sarkari

Mohammad Reza Fakoorziba

Mohsen Kalantari

Mohammad Djaefar Moemenbellah-Fard

Mohsen Ali-Akbarpour

Affiliations

Soon will be listed here.

Abstract

Background: Geographical distribution of zoonotic cutaneous leishmaniasis (ZCL) has continuously been extended in recent years in Iran. The Beiza District is one of the newly-emerged endemic foci of ZCL in southern Iran. The main aim of the present study was to detect the vector(s) of ZCL in this area.

Methods: To detect the fauna and vectors of ZCL in this district, sand flies were caught using sticky papers. Seventy randomly selected female sand flies out of 730 were molecularly investigated for Leishmania infection using species-specific nested polymerase chain reaction (PCR) assay between April and October 2010.

Results: A total of 2543 sand flies were caught. The fauna was identified as 10 species (five Phlebotomus spp. and five Sergentomyia spp.). Phlebotomus papatasi was the most dominant species both indoors and outdoors (37.55% and 16.35 %, respectively). L. major was detected in 5 out of 48 investigated Phlebotomus papatasi (10.41%). Sequence-based characterization was carried out to confirm the PCR findings. The positive samples were shown to have 75-88% similarity with L. major sequences in GenBank.

Conclusion: According to the findings of the present study, similar to the other foci of ZCL in Iran, P. papatasi is the proven and primary vector of CL. This study could be drawn upon for future strategy planning in this newly emerged endemic focus.

Citing Articles

Molecular Survey of Infection of Sand Flies in Karun County, Southwestern Iran.

Taheri S, Jahanifard E, Vazirianzadeh B, Arjmand R J Arthropod Borne Dis. 2024; 18(1):37-46.

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First Report of Natural Infection of to and in the Endemic Foci of Zoonotic Cutaneous Leishmaniasis in Iran.

Absavaran A, Mohebali M, Moin-Vaziri V, Zahraei-Ramazani A, Akhavan A, Rafizadeh S J Arthropod Borne Dis. 2023; 16(4):315-324.

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Nahidi Y, Tayyebi Meibodi N, Ghazvini K, Arabi Moghadam H, Akhlaghi S, Torabian F Iran J Parasitol. 2023; 17(4):573-582.

PMID: 36694566 PMC: 9825699. DOI: 10.18502/ijpa.v17i4.11285.

Cutaneous leishmaniasis in Iran: A review of epidemiological aspects, with emphasis on molecular findings.

Nasiri Z, Kalantari M, Mohammadi J, Daliri S, Mehrabani D, Azizi K Parasite. 2022; 29:47.

PMID: 36269100 PMC: 9585930. DOI: 10.1051/parasite/2022047.

Sandflies species composition, activity, and natural infection with , parasite identity in lesion isolates of cutaneous leishmaniasis, central Iran.

Gholamian-Shahabad M, Azizi K, Asgari Q, Kalantari M, Moemenbellah-Fard M J Parasit Dis. 2018; 42(2):252-258.

PMID: 29844630 PMC: 5962503. DOI: 10.1007/s12639-018-0994-y.

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