Stability and Fitness Impact of the Visually Discernible Rosea1 Marker in the Tobacco Etch Virus Genome

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2013 Sep 12

PMID 24022073

Citations 9

Authors

Eszter Majer

Jose-Antonio Daros

Mark P Zwart

Affiliations

Soon will be listed here.

Abstract

Antirrhinum majus Rosea1 (Ros1) is an MYB-related transcription factor that induces anthocyanin biosynthesis in plant tissues, and has been shown to be suitable for visual tracking of virus infection in plants. However, activation of anthocyanin biosynthesis has far reaching effects on plant physiology and could consequently have negative effects on viral replication. Therefore, viruses carrying the Ros1 marker might have a low fitness and consequently rapidly lose the marker. To compare the stability of the Ros1 marker, we generated Tobacco etch virus (TEV) based constructs containing either Ros1 or the enhanced green fluorescent protein (eGFP) between the NIb and CP cistrons (TEV-Ros1 and TEV-eGFP, respectively). We measured the within-host competitive fitness of both viruses by direct competitions with a common competitor during infection of Nicotiana tabacum. The fitness of TEV-Ros1 was significantly lower than that of TEV-eGFP, and both recombinant viruses had a significantly lower fitness than the wild-type virus. Nevertheless, after seven weeks of infection in N. tabacum, similar levels of marker gene instability where found for both viruses. Despite lower fitness of the marked virus, Ros1 is therefore a viable alternative marker for tracking viral infection in plants.

Citing Articles

On the stability of sequences inserted into viral genomes.

Willemsen A, Zwart M Virus Evol. 2019; 5(2):vez045.

PMID: 31741748 PMC: 6855363. DOI: 10.1093/ve/vez045.

Phloem-Triggered Virus-Induced Gene Silencing Using a Recombinant Polerovirus.

Bortolamiol-Becet D, Monsion B, Chapuis S, Hleibieh K, Scheidecker D, Alioua A Front Microbiol. 2018; 9:2449.

PMID: 30405546 PMC: 6206295. DOI: 10.3389/fmicb.2018.02449.

Going, going, gone: predicting the fate of genomic insertions in plant RNA viruses.

Willemsen A, Carrasco J, Elena S, Zwart M Heredity (Edinb). 2018; 121(5):499-509.

PMID: 29743566 PMC: 6180052. DOI: 10.1038/s41437-018-0086-x.

Rewiring carotenoid biosynthesis in plants using a viral vector.

Majer E, Llorente B, Rodriguez-Concepcion M, Daros J Sci Rep. 2017; 7:41645.

PMID: 28139696 PMC: 5282570. DOI: 10.1038/srep41645.

2b or Not 2b: Experimental Evolution of Functional Exogenous Sequences in a Plant RNA Virus.

Willemsen A, Zwart M, Ambros S, Carrasco J, Elena S Genome Biol Evol. 2017; 9(2):297-310.

PMID: 28137747 PMC: 5381683. DOI: 10.1093/gbe/evw300.

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