Understanding Inositol Pyrophosphate Metabolism and Function: Kinetic Characterization of the DIPPs

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2013 Sep 12

PMID 24021644

Citations 42

Authors

Rajagopal S Kilari

Jeremy D Weaver

Stephen B Shears

Stephen T Safrany

Affiliations

Soon will be listed here.

Abstract

We illuminate the metabolism and the cell-signaling activities of inositol pyrophosphates, by showing that regulation of yeast cyclin-kinase by 1-InsP7 is not conserved for mammalian CDK5, and by kinetically characterizing Ddp1p/DIPP-mediated dephosphorylation of 1-InsP7, 5-InsP7 and InsP8. Each phosphatase exhibited similar Km values for every substrate (range: 35-148 nM). The rank order of kcat values (1-InsP7>5-InsP7=InsP8) was identical for each enzyme, although DIPP1 was 10- to 60-fold more active than DIPP2α/β and DIPP3α/β. We demonstrate InsP8 dephosphorylation preferentially progresses through 1-InsP7. Conversely, we conclude that the more metabolically and functionally significant steady-state route of InsP8 synthesis proceeds via 5-InsP7.

Citing Articles

Inositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development.

Laurent F, Bartsch S, Shukla A, Rico-Resendiz F, Couto D, Fuchs C PLoS Genet. 2024; 20(11):e1011468.

PMID: 39531477 PMC: 11611267. DOI: 10.1371/journal.pgen.1011468.

Using native and synthetic genes to disrupt inositol pyrophosphates and phosphate accumulation in plants.

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Activities and genetic interactions of fission yeast Aps1, a Nudix-type inositol pyrophosphatase and inorganic polyphosphatase.

Ghosh S, Sanchez A, Schwer B, Prucker I, Jork N, Jessen H mBio. 2024; 15(8):e0108424.

PMID: 38940614 PMC: 11323792. DOI: 10.1128/mbio.01084-24.

Protein pyrophosphorylation by inositol phosphates: a novel post-translational modification in plants?.

Mihiret Y, Schaaf G, Kamleitner M Front Plant Sci. 2024; 15:1347922.

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Insights into the roles of inositol hexakisphosphate kinase 1 (IP6K1) in mammalian cellular processes.

Chakkour M, Greenberg M J Biol Chem. 2024; 300(4):107116.

PMID: 38403246 PMC: 11065760. DOI: 10.1016/j.jbc.2024.107116.

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