Effects of Laser Therapy on the Proliferation of Human Periodontal Ligament Stem Cells

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2013 Sep 10

PMID 24013624

Citations 33

Authors

Diego Moura Soares

Fernanda Ginani

Aguida Gomes Henriques

Carlos Augusto Galvao Barboza

Affiliations

Soon will be listed here.

Abstract

Low-level laser irradiation (LLLI) stimulates the proliferation of a variety of cell types. However, very little is known about the effect of laser therapy on dental stem cells. The aim of the present study was to evaluate the effect of LLLI (660 nm, 30 mW) on the proliferation rate of human periodontal ligament stem cells (hPDLSC), obtained from two healthy permanent third molars extracted due to surgical indication. Culture cells were either irradiated or not (control) with an InGaAIP diode laser at 0 and 48 h, using two different energy densities (0.5 J/cm², 16 s and 1.0 J/cm², 33 s). Cell proliferation was evaluated by the Trypan blue exclusion method and by measuring mitochondrial activity using the MTT-based cytotoxicity assay at intervals of 0, 24, 48, and 72 h after the first laser application. An energy density of 1.0 J/cm² improved the cell proliferation in comparison to the other groups (control and laser 0.5 J/cm²) at 48 and 72 h. The group irradiated with 1.0 J/cm² presented significantly higher MTT activity at 48 and 72 h when compared to the energy density of 0.5 J/cm². It can be concluded that LLLI using infrared light and an energy density of 1.0 J/cm² has a positive stimulatory effect on the proliferation of hPDLSC.

Citing Articles

Light assisted modulation of stem cell function and secretome production: a systematic review on current status and new avenues for regenerative medicine.

Rastogi M, Sahu K, Majumder S Lasers Med Sci. 2025; 40(1):83.

PMID: 39934459 PMC: 11814041. DOI: 10.1007/s10103-025-04339-5.

Evaluating the effectiveness of advanced platelet-rich fibrin, photobiomodulation, pentoxifylline, and Alveogyl in the treatment of alveolar osteitis: a randomized controlled clinical trial.

Yuzbasioglu A, Eroglu C BMC Oral Health. 2024; 24(1):1559.

PMID: 39725929 PMC: 11673614. DOI: 10.1186/s12903-024-05372-6.

Effect of nutritional stress and photobiomodulation protocol on in vitro viability and proliferation of murine preosteoblast cells.

Laffitte C, Sabino V, Rosado M, Carvalho V, da Costa Miguel M, de Moura C Lasers Med Sci. 2024; 39(1):289.

PMID: 39643747 DOI: 10.1007/s10103-024-04245-2.

The proliferation and viability of human periodontal ligament stem cells cultured on polymeric scaffolds can be improved by low-level laser irradiation.

Lira J, Sabino V, da Costa E, de Paula J, Rocha H, de Moura C Lasers Med Sci. 2024; 39(1):261.

PMID: 39428431 DOI: 10.1007/s10103-024-04210-z.

Clinical Evaluation of Diode Laser as an Adjunct to Scaling and Root Planing in Patients With Chronic Periodontitis and Type II Diabetes Mellitus: A Split-Mouth Study.

Lalli A, Dhingra S, Lalli A, Kaur G, Gupta H, Goyal S Cureus. 2024; 16(2):e54178.

PMID: 38496103 PMC: 10941804. DOI: 10.7759/cureus.54178.

References

Seo B, Miura M, Gronthos S, Bartold P, Batouli S, Brahim J . Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004; 364(9429):149-55. DOI: 10.1016/S0140-6736(04)16627-0. View

Wu Y, Wang J, Gong D, Gu H, Hu S, Zhang H . Effects of low-level laser irradiation on mesenchymal stem cell proliferation: a microarray analysis. Lasers Med Sci. 2011; 27(2):509-19. DOI: 10.1007/s10103-011-0995-x. View

Fujihara N, Hiraki K, Marques M . Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med. 2006; 38(4):332-6. DOI: 10.1002/lsm.20298. View

Hawkins D, Abrahamse H . Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. Photomed Laser Surg. 2007; 24(6):705-14. DOI: 10.1089/pho.2006.24.705. View

Benedicenti S, Pepe I, Angiero F, Benedicenti A . Intracellular ATP level increases in lymphocytes irradiated with infrared laser light of wavelength 904 nm. Photomed Laser Surg. 2008; 26(5):451-3. DOI: 10.1089/pho.2007.2218. View

Mvula B, Moore T, Abrahamse H . Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells. Lasers Med Sci. 2009; 25(1):33-9. DOI: 10.1007/s10103-008-0636-1. View

Eduardo F, Bueno D, de Freitas P, Marques M, Passos-Bueno M, Eduardo C . Stem cell proliferation under low intensity laser irradiation: a preliminary study. Lasers Surg Med. 2008; 40(6):433-8. DOI: 10.1002/lsm.20646. View

Moore P, Ridgway T, Higbee R, Howard E, Lucroy M . Effect of wavelength on low-intensity laser irradiation-stimulated cell proliferation in vitro. Lasers Surg Med. 2005; 36(1):8-12. DOI: 10.1002/lsm.20117. View

Damante C, Micheli G, Miyagi S, Feist I, Marques M . Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci. 2008; 24(6):885-91. DOI: 10.1007/s10103-008-0582-y. View

10.

de Villiers J, Houreld N, Abrahamse H . Influence of low intensity laser irradiation on isolated human adipose derived stem cells over 72 hours and their differentiation potential into smooth muscle cells using retinoic acid. Stem Cell Rev Rep. 2011; 7(4):869-82. DOI: 10.1007/s12015-011-9244-8. View

11.

Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, Thurnher D . Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr. 2008; 120(3-4):112-7. DOI: 10.1007/s00508-008-0932-6. View

12.

Chen S, Marino V, Gronthos S, Bartold P . Location of putative stem cells in human periodontal ligament. J Periodontal Res. 2006; 41(6):547-53. DOI: 10.1111/j.1600-0765.2006.00904.x. View

13.

Tuby H, Maltz L, Oron U . Low-level laser irradiation (LLLI) promotes proliferation of mesenchymal and cardiac stem cells in culture. Lasers Surg Med. 2007; 39(4):373-8. DOI: 10.1002/lsm.20492. View

14.

Horvat-Karajz K, Balogh Z, Kovacs V, Drrernat A, Sreter L, Uher F . In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med. 2009; 41(6):463-9. DOI: 10.1002/lsm.20791. View

15.

Li W, Leu Y, Wu J . Red-light light-emitting diode irradiation increases the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Photomed Laser Surg. 2010; 28 Suppl 1:S157-65. DOI: 10.1089/pho.2009.2540. View

16.

Huang Y, Chen A, Carroll J, Hamblin M . Biphasic dose response in low level light therapy. Dose Response. 2009; 7(4):358-83. PMC: 2790317. DOI: 10.2203/dose-response.09-027.Hamblin. View

17.

Ozcelik O, Haytac M, Seydaoglu G . Enamel matrix derivative and low-level laser therapy in the treatment of intra-bony defects: a randomized placebo-controlled clinical trial. J Clin Periodontol. 2007; 35(2):147-56. DOI: 10.1111/j.1600-051X.2007.01176.x. View

18.

AboElsaad N, Soory M, Gadalla L, Ragab L, Dunne S, Zalata K . Effect of soft laser and bioactive glass on bone regeneration in the treatment of infra-bony defects (a clinical study). Lasers Med Sci. 2008; 24(3):387-95. DOI: 10.1007/s10103-008-0576-9. View

19.

Gronthos S, Mankani M, Brahim J, Robey P, Shi S . Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97(25):13625-30. PMC: 17626. DOI: 10.1073/pnas.240309797. View

20.

Eduardo F, Mehnert D, Monezi T, Zezell D, Schubert M, Eduardo C . Cultured epithelial cells response to phototherapy with low intensity laser. Lasers Surg Med. 2007; 39(4):365-72. DOI: 10.1002/lsm.20481. View