DNA-directed Self-assembly of Shape-controlled Hydrogels

Overview

Journal Nat Commun

Specialty Biology

Date 2013 Sep 10

PMID 24013352

Citations 61

Authors

Hao Qi

Majid Ghodousi

Yanan Du

Casey Grun

Hojae Bae

Peng Yin

Ali Khademhosseini

Affiliations

Soon will be listed here.

Abstract

Using DNA as programmable, sequence-specific 'glues', shape-controlled hydrogel units are self-assembled into prescribed structures. Here we report that aggregates are produced using hydrogel cubes with edge lengths ranging from 30 μm to 1 mm, demonstrating assembly across scales. In a simple one-pot agitation reaction, 25 dimers are constructed in parallel from 50 distinct hydrogel cube species, demonstrating highly multiplexed assembly. Using hydrogel cuboids displaying face-specific DNA glues, diverse structures are achieved in aqueous and in interfacial agitation systems. These include dimers, extended chains and open network structures in an aqueous system, and dimers, chains of fixed length, T-junctions and square shapes in the interfacial system, demonstrating the versatility of the assembly system.

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