Low-dose Gold Nanoparticles Exert Subtle Endocrine-modulating Effects on the Ovarian Steroidogenic Pathway Ex Vivo Independent of Oxidative Stress

Overview

Journal Nanotoxicology

Publisher Informa Healthcare

Specialties Biotechnology
Toxicology

Date 2013 Sep 3

PMID 23992423

Citations 4

Authors

Jeremy K Larson

Michael J Carvan 3rd

Justin G Teeguarden

Gen Watanabe

Kazuyoshi Taya

Evan Krystofiak

Reinhold J Hutz

Affiliations

Soon will be listed here.

Abstract

Gold nanoparticles (GNPs) have gained considerable attention for application in science and industry. However, the untoward effects of such particles on female fertility remain unclear. The objectives of this study were to (1) examine the effects of 10-nm GNPs on progesterone and estradiol-17β accumulation by rat ovaries ex vivo and (2) to identify the locus/loci whereby GNPs modulate steroidogenesis via multiple-reference gene quantitative real-time RT-PCR. Regression analyses indicated a positive relationship between both Star (p < 0.05, r(2) = 0.278) and Cyp11a1 (p < 0.001, r(2) = 0.366) expression and P4 accumulation upon exposure to 1.43 × 10(6) GNPs/mL. Additional analyses showed that E2 accumulation was positively associated with Hsd3b1 (p < 0.05, r(2) = 0.181) and Cyp17a1 (p < 0.01, r(2) = 0.301) expression upon exposure to 1.43 × 1(3) and 1.43 × 10(9) GNPs/mL, respectively. These results suggest a subtle treatment-dependent impact of low-dose GNPs on the relationship between progesterone or estradiol-17β and specific steroidogenic target genes, independent of oxidative stress or inhibin.

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