Transcriptome Analysis of Litopenaeus Vannamei in Response to White Spot Syndrome Virus Infection

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Aug 31

PMID 23991181

Citations 33

Authors

Xiaohan Chen

Digang Zeng

Xiuli Chen

Daxiang Xie

Yongzhen Zhao

Chunling Yang

Yongmei Li

Ning Ma

Ming Li

Qiong Yang

Zhenping Liao

Hui Wang

Affiliations

Soon will be listed here.

Abstract

Pacific white shrimp (Litopenaeus vannamei) is the most extensively farmed crustacean species in the world. White spot syndrome virus (WSSV) is one of the major pathogens in the cultured shrimp. However, the molecular mechanisms of the host-virus interaction remain largely unknown. In this study, the impact of WSSV infection on host gene expression in the hepatopancreas of L. vannamei was investigated through the use of 454 pyrosequencing-based RNA-Seq of cDNA libraries developed from WSSV-challenged shrimp or normal controls. By comparing the two cDNA libraries, we show that 767 host genes are significantly up-regulated and 729 genes are significantly down-regulated by WSSV infection. KEGG analysis of the differentially expressed genes indicated that the distribution of gene pathways between the up- and down-regulated genes is quite different. Among the differentially expressed genes, several are found to be involved in various processes of animal defense against pathogens such as apoptosis, mitogen-activated protein kinase (MAPK) signaling, toll-like receptor (TLR) signaling, Wnt signaling and antigen processing and presentation pathways. The present study provides valuable information on differential expression of L. vannamei genes following WSSV infection and improves our current understanding of this host-virus interaction. In addition, the large number of transcripts obtained in this study provides a strong basis for future genomic research on shrimp.

Citing Articles

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