The Cryo-needle: a New Tool for Histological Biopsies. A Feasibility Study

Overview

Journal Lung

Specialty Pulmonary Medicine

Date 2013 Aug 31

PMID 23990134

Citations 8

Authors

Karl-Josef Franke

Georg Nilius

Karl-Heinz Ruehle

Markus D Enderle

Walter Linzenbold

Claus Hann von Weyhern

Affiliations

Soon will be listed here.

Abstract

Purpose: Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a standard procedure for intrathoracic lymph node biopsies. The newly developed cryo-needle operates in a similar way to the EBUS-TBNA but is able to obtain specimens for histological evaluation. The purpose of this animal study was to evaluate the feasibility, effect, and safety of the cryo-needle biopsies.

Methods: Four EBUS-guided cryo-needle biopsies were obtained from a mediastinal lymph node of a healthy pig. In an open surgery approach, cryo-needle biopsies using activation times of 1, 2, and 3 s (A1/A2/A3) and needle biopsies using a 21-gauge EBUS-TBNA needle were obtained from mesenteric lymph nodes. Cryo-needle biopsies A2 were performed with (A2+) and without (A2-) an oversheath. The size, weight, percentage of lymphatic tissue and artefact-free area of each cryobiopsy were evaluated. Smears were made with the TBNA-needle aspirates to determine the number of lymphocytes per high-power field (HPF). The bleeding duration was measured.

Results: We successfully obtained EBUS-guided cryo-needle biopsies. The area and weight of the biopsies A3 and A2+ were significantly larger compared with A1 (1.7 ± 0.8 and 1.4 ± 0.3 vs. 0.9 ± 0.4 mm(2); 5.2 ± 2.4 and 3.4 ± 1.8 vs. 1.5 ± 0.7 mg). The percentage of lymphatic tissue of the cryobiopsies was 90 ± 25 and 98 % of samples were artefact-free. The number of lymphocytes/HPF of TBNA-needle smears was 128 ± 54.3. There was no difference in bleeding duration between the techniques.

Conclusions: The cryo-needle yields large histological specimens of high quality.

Citing Articles

Ultrasound-guided transbronchial cryobiopsy of mediastinal and hilar lesions: a multicenter pragmatic cohort study with real-world evidence.

Mangold M, Franzen D, Hetzel J, Latshang T, Roeder M, Vesenbeckh S BMJ Open Respir Res. 2024; 11(1.

PMID: 39668107 PMC: 11647314. DOI: 10.1136/bmjresp-2024-002617.

Meta-analysis and systematic review of mediastinal cryobiopsy versus endobronchial ultrasound-transbronchial needle aspiration (EBUS-TBNA) in the diagnosis of intrathoracic adenopathy.

Mathew R, Roy W, Thomas E, Meena N, Danilevskaya O J Thorac Dis. 2024; 16(7):4217-4228.

PMID: 39144333 PMC: 11320235. DOI: 10.21037/jtd-24-348.

An explorative analysis on the optimal cryo-passes and freezing time of the ultrathin cryoprobe in endobronchial ultrasound-guided transbronchial mediastinal cryobiopsy.

Kho S, Tan S, Soo C, Ramarmuty H, Chai C, Huan N Sci Rep. 2024; 14(1):18653.

PMID: 39134712 PMC: 11319585. DOI: 10.1038/s41598-024-69702-y.

Cryo-Biopsy versus 19G needle versus 22G needle with EBUS-TBNA endoscopy.

Oikonomidou R, Petridis D, Kosmidis C, Sapalidis K, Hohenforst-Schmidt W, Christakidis V J Cancer. 2022; 13(10):3084-3090.

PMID: 36046658 PMC: 9414011. DOI: 10.7150/jca.75589.

Radial-EBUS: CryoBiopsy Versus Conventional Biopsy: Time-Sample and C-Arm.

Zarogoulidis P, Kosmidis C, Hohenforst-Schmidt W, Matthaios D, Sapalidis K, Petridis D Int J Environ Res Public Health. 2022; 19(6).

PMID: 35329255 PMC: 8955438. DOI: 10.3390/ijerph19063569.

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