Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2013 Aug 28

PMID 23979477

Citations 42

Authors

Johann Gout

Roxane M Pommier

David F Vincent

Bastien Kaniewski

Sylvie Martel

Ulrich Valcourt

Laurent Bartholin

Affiliations

Soon will be listed here.

Abstract

This protocol permits rapid isolation (in less than 1 hr) of murine pancreatic acini, making it possible to maintain them in culture for more than one week. More than 20 x 10(6) acinar cells can be obtained from a single murine pancreas. This protocol offers the possibility to independently process as many as 10 pancreases in parallel. Because it preserves acinar architecture, this model is well suited for studying the physiology of the exocrine pancreas in vitro in contrast to cell lines established from pancreatic tumors, which display many genetic alterations resulting in partial or total loss of their acinar differentiation.

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