Identification of Novel α-n-methylation of CENP-B That Regulates Its Binding to the Centromeric DNA

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2013 Aug 28

PMID 23978223

Citations 44

Authors

Xiaoxia Dai

Koichiro Otake

Changjun You

Qian Cai

Zi Wang

Hiroshi Masumoto

Yinsheng Wang

Affiliations

Soon will be listed here.

Abstract

The eukaryotic centromere is an essential chromatin region required for accurate segregation of sister chromatids during cell division. Centromere protein B (CENP-B) is a highly conserved protein which can bind to the 17-bp CENP-B box on the centromeric DNA. In this study, we found that CENP-B could be α-N-methylated in human cells. We also showed that the level of the α-N-methylation was stimulated in cells in response to a variety of extracellular stimuli, including increased cell density, heat shock, and arsenite treatment, although the methylation level was not altered upon metaphase arrest. We identified N-terminal RCC1 methyltransferase (NRMT) as a major enzyme required for the CENP-B methylation. Additionally, we found that chromatin-bound CENP-B was primarily trimethylated and α-N-trimethylation could enhance CENP-B's binding to CENP-B box in cells. Our study also expands the function of protein α-N-methylation that has been known for decades and whose function remains largely unexplored.

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