Purification and Heterogeneity of Inorganic Pyrophosphatase of Pig Scapula Cartilage

Overview

Journal Biochem J

Specialty Biochemistry

Date 1975 Apr 1

PMID 239695

Citations 7

Authors

R Felix

H Fleisch

Affiliations

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Abstract

Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.

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