Molecular Genotyping of Toxoplasma Gondii in Human Spontaneous Aborted Fetuses in Shiraz, Southern Iran

Overview

Journal Iran J Public Health

Date 2013 Aug 23

PMID 23967430

Citations 19

Authors

Qasem Asgari

Maysam Fekri

Ahmad Monabati

Mohsen Kalantary

Iraj Mohammadpour

Mohammad Hossein Motazedian

Bahador Sarkari

Affiliations

Soon will be listed here.

Abstract

Background: Congenital toxoplasmosis is associated with variable complications including encephalitis, microcephaly, hydrocephaly, hepatitis, lymphadenopathy and even intrauterine death. Presence of Toxoplasma gondii in human placenta may induce congenital infection. The aim of this study was to determine the genotypes of Toxoplasma gondii infection in human spontaneous aborted fetuses in Shiraz, south of Iran.

Methods: Five hundred and forty two paraffin-embedded blocks of aborted placenta were collected, from two university-affiliated hospitals in Shiraz. Occurrence of spontaneous abortion was confirmed by examine of the slides. After re-cutting of the blocks and dewaxing, semi-nested PCR assay was used to detect the fragments of T. gondii B1 gene in the samples. Also direct molecular genotyping was performed on positive samples with Restriction Fragment Length Polymorphism-PCR analysis on the SAG2 gene.

Results: Among the 542 tissue samples, the B1 gene was amplified from 78 (14.4%) of cases with the semi nested PCR and typed by RFLP. The genotype of Toxoplasma strains of 65 (out of 78) PCR-positive samples were evaluated and 54 out of 65 (83.1%) were found to be type II and 11 out of 65 (16.9%) were type I.

Conclusion: Considering the high level of Toxoplasma infection in aborted fetuses in this study, Toxoplasma might largely contribute to spontaneous abortion in this area of Iran.

Citing Articles

Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran.

Zeinali S, Khademvatan S, Jafari R, Vazifekhah S, Yousefi E, Behroozi-Lak T PLoS One. 2023; 18(10):e0283493.

PMID: 37883415 PMC: 10602335. DOI: 10.1371/journal.pone.0283493.

More Than Seventy Years of Research (1948-November 2021) on in Iran: A Narrative Review.

Sadeghi M, Hosseini S, Sarvi S, Nayeri T, Sharif M, Pagheh A Iran J Parasitol. 2022; 17(2):124-137.

PMID: 36032751 PMC: 9363252. DOI: 10.18502/ijpa.v17i2.9528.

Detection and phylogenetic analysis of and spp. (Sarcocystidae: Apicomplexa) from slaughtered sheep in southwest Iran.

Shahabi S, Dehbashi N, Sarkari B, ArefKhah N, Sedaghat B, Savardashtaki A J Parasit Dis. 2022; 46(1):215-219.

PMID: 35299931 PMC: 8901842. DOI: 10.1007/s12639-021-01433-7.

Detection of Acute and Chronic Infection among Women with History of Abortion in the Southwest of Iran.

Saki J, Zamanpour M, Najafian M, Mohammadpour N, Foroutan M J Parasitol Res. 2021; 2021:6693070.

PMID: 34765259 PMC: 8577945. DOI: 10.1155/2021/6693070.

Molecular Genotyping of in Sheep Aborted Fetuses Reveals Predominance of Type I Infection in Southwest of Iran.

ArefKhah N, Sarkari B, Asgari Q, Moshfe A, Khalafi M, Mohammadpour I Iran J Parasitol. 2020; 15(3):374-382.

PMID: 33082802 PMC: 7548476. DOI: 10.18502/ijpa.v15i3.4202.

References

Dentico P, Volpe A, Putoto G, Ramadani N, Bertinato L, Berisha M . Toxoplasmosis in Kosovo pregnant women. New Microbiol. 2011; 34(2):203-7. View

KIMBALL A, KEAN B, Fuchs F . Congenital toxoplasmosis: a prospective study of 4,048 obstetric patients. Am J Obstet Gynecol. 1971; 111(2):211-8. DOI: 10.1016/0002-9378(71)90892-1. View

Assmar M, Amirkhani A, Piazak N, Hovanesian A, Kooloobandi A, Etessami R . [Toxoplasmosis in Iran. Results of a seroepidemiological study]. Bull Soc Pathol Exot. 1997; 90(1):19-21. View

Fekkar A, Ajzenberg D, Bodaghi B, Touafek F, Le Hoang P, Delmas J . Direct genotyping of Toxoplasma gondii in ocular fluid samples from 20 patients with ocular toxoplasmosis: predominance of type II in France. J Clin Microbiol. 2011; 49(4):1513-7. PMC: 3122785. DOI: 10.1128/JCM.02196-10. View

Gomez-Marin J, de-la-Torre A, Angel-Muller E, Rubio J, Arenas J, Osorio E . First Colombian multicentric newborn screening for congenital toxoplasmosis. PLoS Negl Trop Dis. 2011; 5(5):e1195. PMC: 3104965. DOI: 10.1371/journal.pntd.0001195. View

Ajzenberg D, Cogne N, Paris L, Bessieres M, Thulliez P, Filisetti D . Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings. J Infect Dis. 2002; 186(5):684-9. DOI: 10.1086/342663. View

Dunn D, Wallon M, Peyron F, Petersen E, Peckham C, Gilbert R . Mother-to-child transmission of toxoplasmosis: risk estimates for clinical counselling. Lancet. 1999; 353(9167):1829-33. DOI: 10.1016/S0140-6736(98)08220-8. View

Saeedi M, Veghari G, Marjani A . Seroepidemiologic evaluation of anti-toxoplasma antibodies among women in north of Iran. Pak J Biol Sci. 2008; 10(14):2359-62. DOI: 10.3923/pjbs.2007.2359.2362. View

Montoya J, Liesenfeld O . Toxoplasmosis. Lancet. 2004; 363(9425):1965-76. DOI: 10.1016/S0140-6736(04)16412-X. View

10.

Abdel-Hameed D, Hassanein O . Genotyping of Toxoplasma gondii strains from female patients with toxoplasmosis. J Egypt Soc Parasitol. 2008; 38(2):511-20. View

11.

Hill D, Chirukandoth S, Dubey J, Lunney J, Gamble H . Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine. Vet Parasitol. 2006; 141(1-2):9-17. DOI: 10.1016/j.vetpar.2006.05.008. View

12.

Nowakowska D, Colon I, Remington J, Grigg M, Golab E, Wilczynski J . Genotyping of Toxoplasma gondii by multiplex PCR and peptide-based serological testing of samples from infants in Poland diagnosed with congenital toxoplasmosis. J Clin Microbiol. 2006; 44(4):1382-9. PMC: 1448688. DOI: 10.1128/JCM.44.4.1382-1389.2006. View

13.

Messaritakis I, Detsika M, Koliou M, Sifakis S, Antoniou M . Prevalent genotypes of Toxoplasma gondii in pregnant women and patients from Crete and Cyprus. Am J Trop Med Hyg. 2008; 79(2):205-9. View

14.

Daffos F, Forestier F, Thulliez P, Aufrant C, Valenti D, Cox W . Prenatal management of 746 pregnancies at risk for congenital toxoplasmosis. N Engl J Med. 1988; 318(5):271-5. DOI: 10.1056/NEJM198802043180502. View

15.

Howe D, Sibley L . Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease. J Infect Dis. 1995; 172(6):1561-6. DOI: 10.1093/infdis/172.6.1561. View

16.

Switaj K, Master A, Borkowski P, Skrzypczak M, Wojciechowicz J, Zaborowski P . Association of ocular toxoplasmosis with type I Toxoplasma gondii strains: direct genotyping from peripheral blood samples. J Clin Microbiol. 2006; 44(11):4262-4. PMC: 1698313. DOI: 10.1128/JCM.01786-06. View