» Articles » PMID: 23962819

Iron Sensing and Regulation in Saccharomyces Cerevisiae: Ironing out the Mechanistic Details

Overview
Specialty Microbiology
Date 2013 Aug 22
PMID 23962819
Citations 61
Authors
Affiliations
Soon will be listed here.
Abstract

Regulation of iron metabolism in Saccharomyces cerevisiae is achieved at the transcriptional level by low (Aft1 and Aft2) and high iron-sensing (Yap5) transcription factors, and at the post-transcriptional level by mRNA-binding proteins (Cth1 and Cth2). In this review we highlight recent studies unveiling the critical role that iron-sulfur clusters play in control of Aft1/2 and Yap5 activity, as well as the complex relationship between iron homeostasis and thiol redox metabolism. In addition, new insights into the localization and regulation of Cth1/Cth2 have added another layer of complexity to the cell's adaptation to iron deficiency. Finally, biophysical studies on subcellular iron speciation changes in response to environmental and genetic factors have further illuminated the elaborate control mechanisms required to manage iron bioavailability in the cell.

Citing Articles

Siderophore Biosynthesis and Transport Systems in Model and Pathogenic Fungi.

Choi S, Kronstad J, Jung W J Microbiol Biotechnol. 2024; 34(8):1551-1562.

PMID: 38881181 PMC: 11380514. DOI: 10.4014/jmb.2405.05020.


The Oxidative Stress Response Highly Depends on Glucose and Iron Availability in .

Emri T, Antal K, Varga K, Gila B, Pocsi I J Fungi (Basel). 2024; 10(3).

PMID: 38535229 PMC: 10970741. DOI: 10.3390/jof10030221.


Glutathione and Glutaredoxin-Key Players in Cellular Redox Homeostasis and Signaling.

Chai Y, Mieyal J Antioxidants (Basel). 2023; 12(8).

PMID: 37627548 PMC: 10451691. DOI: 10.3390/antiox12081553.


Aft1 Nuclear Localization and Transcriptional Response to Iron Starvation Rely upon TORC2/Ypk1 Signaling and Sphingolipid Biosynthesis.

Montella-Manuel S, Pujol-Carrion N, de la Torre-Ruiz M Int J Mol Sci. 2023; 24(3).

PMID: 36768760 PMC: 9916926. DOI: 10.3390/ijms24032438.


Yeast cells depleted of the frataxin homolog Yfh1 redistribute cellular iron: Studies using Mössbauer spectroscopy and mathematical modeling.

Fernandez S, Wofford J, Shepherd R, Vali S, Dancis A, Lindahl P J Biol Chem. 2022; 298(6):101921.

PMID: 35413285 PMC: 9130540. DOI: 10.1016/j.jbc.2022.101921.


References
1.
Hausmann A, Samans B, Lill R, Muhlenhoff U . Cellular and mitochondrial remodeling upon defects in iron-sulfur protein biogenesis. J Biol Chem. 2008; 283(13):8318-30. DOI: 10.1074/jbc.M705570200. View

2.
Li L, Kaplan J . A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism. J Biol Chem. 2004; 279(32):33653-61. DOI: 10.1074/jbc.M403146200. View

3.
Li L, Bagley D, Ward D, Kaplan J . Yap5 is an iron-responsive transcriptional activator that regulates vacuolar iron storage in yeast. Mol Cell Biol. 2007; 28(4):1326-37. PMC: 2258748. DOI: 10.1128/MCB.01219-07. View

4.
Ueta R, Fujiwara N, Iwai K, Yamaguchi-Iwai Y . Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Mol Cell Biol. 2012; 32(24):4998-5008. PMC: 3510542. DOI: 10.1128/MCB.00726-12. View

5.
Cockrell A, Holmes-Hampton G, McCormick S, Chakrabarti M, Lindahl P . Mössbauer and EPR study of iron in vacuoles from fermenting Saccharomyces cerevisiae. Biochemistry. 2011; 50(47):10275-83. PMC: 3226750. DOI: 10.1021/bi2014954. View