Screening of Cell Cycle-Related Genes of Pleurotus Eryngii Using Yeast Mutant Strains

Overview

Journal Mycobiology

Date 2013 Aug 20

PMID 23956629

Authors

Shanliang Shi

Hyeon-Su Ro

Affiliations

Soon will be listed here.

Abstract

Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over 30℃. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from 30℃ to 37℃. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.

References

Visintin R, Stegmeier F, Amon A . The role of the polo kinase Cdc5 in controlling Cdc14 localization. Mol Biol Cell. 2003; 14(11):4486-98. PMC: 266767. DOI: 10.1091/mbc.e03-02-0095. View

Rodriguez Estrada A, Jimenez-Gasco M, Royse D . Improvement of yield of Pleurotus eryngii var. eryngii by substrate supplementation and use of a casing overlay. Bioresour Technol. 2009; 100(21):5270-6. DOI: 10.1016/j.biortech.2009.02.073. View

Monje-Casas F, Amon A . Cell polarity determinants establish asymmetry in MEN signaling. Dev Cell. 2009; 16(1):132-45. PMC: 2713012. DOI: 10.1016/j.devcel.2008.11.002. View

Rahal R, Amon A . The Polo-like kinase Cdc5 interacts with FEAR network components and Cdc14. Cell Cycle. 2008; 7(20):3262-72. PMC: 2665935. DOI: 10.4161/cc.7.20.6852. View

Darieva Z, Bulmer R, Pic-Taylor A, Doris K, Geymonat M, Sedgwick S . Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature. 2006; 444(7118):494-8. PMC: 1890309. DOI: 10.1038/nature05339. View

Katoh Y, Katoh M . Hedgehog target genes: mechanisms of carcinogenesis induced by aberrant hedgehog signaling activation. Curr Mol Med. 2009; 9(7):873-86. DOI: 10.2174/156652409789105570. View

Geymonat M, Spanos A, Walker P, Johnston L, Sedgwick S . In vitro regulation of budding yeast Bfa1/Bub2 GAP activity by Cdc5. J Biol Chem. 2003; 278(17):14591-4. DOI: 10.1074/jbc.C300059200. View

Ro H, Song S, Lee K . Bfa1 can regulate Tem1 function independently of Bub2 in the mitotic exit network of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002; 99(8):5436-41. PMC: 122787. DOI: 10.1073/pnas.062059999. View

Gietz R, Woods R . Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol. 2002; 350:87-96. DOI: 10.1016/s0076-6879(02)50957-5. View

10.

Konig C, Maekawa H, Schiebel E . Mutual regulation of cyclin-dependent kinase and the mitotic exit network. J Cell Biol. 2010; 188(3):351-68. PMC: 2819678. DOI: 10.1083/jcb.200911128. View

11.

Stajic M, Vukojevic J, Duletic-Lausevic S . Biology of Pleurotus eryngii and role in biotechnological processes: a review. Crit Rev Biotechnol. 2009; 29(1):55-66. DOI: 10.1080/07388550802688821. View

12.

Nurse P . Cyclin dependent kinases and cell cycle control (nobel lecture). Chembiochem. 2002; 3(7):596-603. DOI: 10.1002/1439-7633(20020703)3:7<596::AID-CBIC596>3.0.CO;2-U. View

13.

Choi U, Bajpai V, Lee N . Influence of calcinated starfish powder on growth, yield, spawn run and primordial germination of king oyster mushroom (Pleurotus eryngii). Food Chem Toxicol. 2009; 47(11):2830-3. DOI: 10.1016/j.fct.2009.08.019. View