» Articles » PMID: 23954469

DNA Oxidative Damage in Mammalian Spermatozoa: Where and Why is the Male Nucleus Affected?

Overview
Date 2013 Aug 20
PMID 23954469
Citations 35
Authors
Affiliations
Soon will be listed here.
Abstract

Gamete DNA integrity is one key parameter conditioning reproductive success as well as the quality of life for the offspring. In particular, damage to the male nucleus can have profound negative effects on the outcome of fertilization. Because of the absence of repair activity of the quiescent mature spermatozoa it is easily subjected to nuclear damage, of which oxidative damage is by far the most prominent. In relation to the organization of the mammalian sperm nucleus we show here that one can correlate the nuclear regions of lower compaction with areas preferentially showing oxidative damage. More precisely, we show that oxidative DNA damage targets primarily histone-rich and nuclear matrix-attached domains located in the peripheral and basal regions of the mouse sperm nucleus. These particular sperm DNA domains were recently shown to be enriched in genes of paramount importance in postfertilization DNA replication events and in the onset of the embryonic developmental program. We propose that monitoring of sperm DNA oxidation using the type of assay presented here should be considered in clinical practice when one wants to estimate the integrity of the paternal nucleus along with more classical assays that essentially analyze DNA fragmentation and nucleus compaction.

Citing Articles

Methods Used to Assess Bull Sperm Chromatin Integrity and Its Correlation with In Vitro Embryo Production Efficiency.

Silva M, Rocha L, Travencolo B, Guimaraes E, Beletti M Vet Sci. 2025; 12(2).

PMID: 40005943 PMC: 11861920. DOI: 10.3390/vetsci12020184.


New horizons in human sperm selection for assisted reproduction.

Nixon B, Schjenken J, Burke N, Skerrett-Byrne D, Hart H, De Iuliis G Front Endocrinol (Lausanne). 2023; 14:1145533.

PMID: 36909306 PMC: 9992892. DOI: 10.3389/fendo.2023.1145533.


A shared 'vulnerability code' underpins varying sources of DNA damage throughout paternal germline transmission in mouse.

Burden F, Ellis P, Farre M Nucleic Acids Res. 2023; 51(5):2319-2332.

PMID: 36806949 PMC: 10018361. DOI: 10.1093/nar/gkad089.


Determination of Ram () Sperm DNA Damage Due to Oxidative Stress: 8-OHdG Immunodetection Assay vs. SCSA.

Soria-Meneses P, Jurado-Campos A, Gomez-Rubio V, Sanchez-Ajofrin I, Soler A, Garde J Animals (Basel). 2022; 12(23).

PMID: 36496807 PMC: 9737133. DOI: 10.3390/ani12233286.


Impacts of oxidative stress on bovine sperm function and subsequent embryo development.

Bollwein H, Bittner L Anim Reprod. 2022; 15(Suppl 1):703-710.

PMID: 36249836 PMC: 9536048. DOI: 10.21451/1984-3143-AR2018-0041.