Cell Survival During Complete Nutrient Deprivation Depends on Lipid Droplet-fueled β-oxidation of Fatty Acids

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2013 Aug 14

PMID 23940052

Citations 93

Authors

Ainara G Cabodevilla

Laura Sanchez-Caballero

Eleni Nintou

Violeta G Boiadjieva

Fernando Picatoste

Albert Gubern

Enrique Claro

Affiliations

Soon will be listed here.

Abstract

Cells exposed to stress of different origins synthesize triacylglycerols and generate lipid droplets (LD), but the physiological relevance of this response is uncertain. Using complete nutrient deprivation of cells in culture as a simple model of stress, we have addressed whether LD biogenesis has a protective role in cells committed to die. Complete nutrient deprivation induced the biogenesis of LD in human LN18 glioblastoma and HeLa cells and also in CHO and rat primary astrocytes. In all cell types, death was associated with LD depletion and was accelerated by blocking LD biogenesis after pharmacological inhibition of Group IVA phospholipase A2 (cPLA2α) or down-regulation of ceramide kinase. Nutrient deprivation also induced β-oxidation of fatty acids that was sensitive to cPLA2α inhibition, and cell survival in these conditions became strictly dependent on fatty acid catabolism. These results show that, during nutrient deprivation, cell viability is sustained by β-oxidation of fatty acids that requires biogenesis and mobilization of LD.

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