Japanese Encephalitis Virus Infects Porcine Kidney Epithelial PK15 Cells Via Clathrin- and Cholesterol-dependent Endocytosis

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2013 Aug 14

PMID 23937769

Citations 25

Authors

Songbai Yang

Minhui He

Xiangdong Liu

Xinyun Li

Bin Fan

Shuhong Zhao

Affiliations

Soon will be listed here.

Abstract

Background: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute viral encephalitis in humans. Pigs are important amplifiers of JEV. The entry mechanism of JEV into porcine cells remains largely unknown. In this study, we present a study of the internalization mechanism of JEV in porcine kidney epithelial PK15 cells.

Results: We demonstrated that the disruption of the lipid raft by cholesterol depletion with methyl-β-cyclodextrin (MβCD) reduced JEV infection. We also found that the knockdown of clathrin by small interfering RNA (siRNA) significantly reduced JEV-infected cells and JEV E-glycoprotein levels, suggesting that JEV utilizes clathrin-dependent endocytosis. In contrast, the knockdown of caveolin-1, a principal component of caveolae, had only a small (although statistically significant) effect on JEV infection, however, JEV entry was not affected by genistein. These results suggested that JEV entry was independent of caveolae.

Conclusions: Taken together, our results demonstrate that JEV enters porcine kidney epithelial PK15 cells through cholesterol- and clathrin-mediated endocytosis.

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