Applications of Small Molecule Probes in Dissecting Mechanisms of Bacterial Virulence and Host Responses

Overview

Journal Biochemistry

Specialty Biochemistry

Date 2013 Aug 14

PMID 23937332

Citations 7

Authors

Aaron W Puri

Matthew Bogyo

Affiliations

Soon will be listed here.

Abstract

Elucidating the molecular and biochemical details of bacterial infections can be challenging because of the many complex interactions that exist between a pathogen and its host. Consequently, many tools have been developed to aid the study of bacterial pathogenesis. Small molecules are a valuable complement to traditional genetic techniques because they can be used to rapidly perturb genetically intractable systems and to monitor post-translationally regulated processes. Activity-based probes are a subset of small molecules that covalently label an enzyme of interest based on its catalytic mechanism. These tools allow monitoring of enzyme activation within the context of a native biological system and can be used to dissect the biochemical details of enzyme function. This review describes the development and application of activity-based probes for examining aspects of bacterial infection on both sides of the host-pathogen interface.

Citing Articles

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