Incretin Response to a Standard Test Meal in a Rat Model of Sleeve Gastrectomy with Diet-induced Obesity

Overview

Journal Obes Surg

Date 2013 Aug 13

PMID 23934273

Citations 6

Authors

Suleiman Al-Sabah

Fahad Alasfar

Ghanim Al-Khaledi

Reshma Dinesh

Mervat Al-Saleh

Habib Abul

Affiliations

Soon will be listed here.

Abstract

Background: Currently, the most effective treatment for obesity is bariatric surgery. Gastroduodenal bypass surgery produces sustained weight loss and improves glycemic control and insulin sensitivity. Previous studies have shown that sleeve gastrectomy (SG) produces similar results and implicate changes in incretin hormone release in these effects.

Methods: Male Sprague-Dawley rats were divided into four groups; lean control (lean), diet-induced obesity (DIO), DIO animals that had undergone SG (SG), and DIO animals that had undergone a sham operation (sham).

Results: After a 2-week recovery period, the incretin response to a standard test meal was measured. Blood sampling was performed in free-moving rats at various time points using chronic vascular access to the right jugular vein. There was a significant increase in the bodyweight of DIO animals fed a high-fat/high-sugar diet compared with the lean animals, which was reversed by SG. DIO caused an impairment of the GLP-1 response to a standard test meal, but not the GIP response. SG resulted in a dramatic increase in the GLP-1 response to a standard test meal but had no effect on the GIP response.

Conclusions: A rapid rise in blood sugar was observed in the SG group following a standard test meal that was followed by reactive hypoglycemia. SG dramatically increases the GLP-1 response to a standard test meal but has no effect on GIP in a rat model of DIO.

Citing Articles

Increased Glucagon Immunoreactivity in a Rat Model of Diet-induced Obesity following Sleeve Gastrectomy.

Al-Sabah S, Jamal M, Al-Khaledi G, Dsouza C, AlOtaibi F, Al-Ali W Med Princ Pract. 2023; .

PMID: 37634505 PMC: 10659591. DOI: 10.1159/000533746.

Vertical sleeve gastrectomy normalizes circulating glucocorticoid levels and lowers glucocorticoid action tissue-selectively in mice.

Akalestou E, Lopez-Noriega L, Christakis I, Hu M, Miras A, Leclerc I Front Endocrinol (Lausanne). 2022; 13:1020576.

PMID: 36246869 PMC: 9556837. DOI: 10.3389/fendo.2022.1020576.

Intravital imaging of islet Ca dynamics reveals enhanced β cell connectivity after bariatric surgery in mice.

Akalestou E, Suba K, Lopez-Noriega L, Georgiadou E, Chabosseau P, Gallie A Nat Commun. 2021; 12(1):5165.

PMID: 34453049 PMC: 8397709. DOI: 10.1038/s41467-021-25423-8.

Short-Term Results Suggest That Sleeved Stomach without Resection Is as Effective as Sleeve Gastrectomy in Improving Glucose Control in Type 2 Diabetes Mellitus Sprague-Dawley Rat Model.

Zhang W, Widjaja J, Yao L, Shao Y, Zhu X, Li C Biomed Res Int. 2020; 2020:9024923.

PMID: 32462030 PMC: 7212312. DOI: 10.1155/2020/9024923.

Role of aquaporin-7 in ghrelin- and GLP-1-induced improvement of pancreatic β-cell function after sleeve gastrectomy in obese rats.

Mendez-Gimenez L, Becerril S, Camoes S, da Silva I, Rodrigues C, Moncada R Int J Obes (Lond). 2017; 41(9):1394-1402.

PMID: 28584298 DOI: 10.1038/ijo.2017.135.

References

Martire S, Holmes N, Westbrook R, Morris M . Altered feeding patterns in rats exposed to a palatable cafeteria diet: increased snacking and its implications for development of obesity. PLoS One. 2013; 8(4):e60407. PMC: 3614998. DOI: 10.1371/journal.pone.0060407. View

Donnelly D . The structure and function of the glucagon-like peptide-1 receptor and its ligands. Br J Pharmacol. 2011; 166(1):27-41. PMC: 3415635. DOI: 10.1111/j.1476-5381.2011.01687.x. View

Nauck M, Heimesaat M, Orskov C, Holst J, Ebert R, Creutzfeldt W . Preserved incretin activity of glucagon-like peptide 1 [7-36 amide] but not of synthetic human gastric inhibitory polypeptide in patients with type-2 diabetes mellitus. J Clin Invest. 1993; 91(1):301-7. PMC: 330027. DOI: 10.1172/JCI116186. View

Romero F, Nicolau J, Flores L, Casamitjana R, Ibarzabal A, Lacy A . Comparable early changes in gastrointestinal hormones after sleeve gastrectomy and Roux-En-Y gastric bypass surgery for morbidly obese type 2 diabetic subjects. Surg Endosc. 2012; 26(8):2231-9. DOI: 10.1007/s00464-012-2166-y. View

Laferrere B, Heshka S, Wang K, Khan Y, Mcginty J, Teixeira J . Incretin levels and effect are markedly enhanced 1 month after Roux-en-Y gastric bypass surgery in obese patients with type 2 diabetes. Diabetes Care. 2007; 30(7):1709-16. PMC: 2743330. DOI: 10.2337/dc06-1549. View

Carr R, Larsen M, Winzell M, Jelic K, Lindgren O, Deacon C . Incretin and islet hormonal responses to fat and protein ingestion in healthy men. Am J Physiol Endocrinol Metab. 2008; 295(4):E779-84. DOI: 10.1152/ajpendo.90233.2008. View

Chambers A, Stefater M, Wilson-Perez H, Jessen L, Sisley S, Ryan K . Similar effects of roux-en-Y gastric bypass and vertical sleeve gastrectomy on glucose regulation in rats. Physiol Behav. 2011; 105(1):120-3. PMC: 3852684. DOI: 10.1016/j.physbeh.2011.05.026. View

Peterli R, Wolnerhanssen B, Peters T, Devaux N, Kern B, Christoffel-Courtin C . Improvement in glucose metabolism after bariatric surgery: comparison of laparoscopic Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy: a prospective randomized trial. Ann Surg. 2009; 250(2):234-41. DOI: 10.1097/SLA.0b013e3181ae32e3. View

Tack J, Arts J, Caenepeel P, De Wulf D, Bisschops R . Pathophysiology, diagnosis and management of postoperative dumping syndrome. Nat Rev Gastroenterol Hepatol. 2009; 6(10):583-90. DOI: 10.1038/nrgastro.2009.148. View

10.

Diehl K, Hull R, Morton D, Pfister R, Rabemampianina Y, Smith D . A good practice guide to the administration of substances and removal of blood, including routes and volumes. J Appl Toxicol. 2001; 21(1):15-23. DOI: 10.1002/jat.727. View

11.

Naslund E, Gryback P, Backman L, Jacobsson H, Holst J, Theodorsson E . Distal small bowel hormones: correlation with fasting antroduodenal motility and gastric emptying. Dig Dis Sci. 1998; 43(5):945-52. DOI: 10.1023/a:1018806129102. View

12.

Zhou J, Livak M, Bernier M, Muller D, Carlson O, Elahi D . Ubiquitination is involved in glucose-mediated downregulation of GIP receptors in islets. Am J Physiol Endocrinol Metab. 2007; 293(2):E538-47. PMC: 2640485. DOI: 10.1152/ajpendo.00070.2007. View

13.

Korner J, Bessler M, Inabnet W, Taveras C, Holst J . Exaggerated glucagon-like peptide-1 and blunted glucose-dependent insulinotropic peptide secretion are associated with Roux-en-Y gastric bypass but not adjustable gastric banding. Surg Obes Relat Dis. 2007; 3(6):597-601. PMC: 2134840. DOI: 10.1016/j.soard.2007.08.004. View

14.

Holst J, Schwartz T, Lovgreen N, Pedersen O, Beck-Nielsen H . Diurnal profile of pancreatic polypeptide, pancreatic glucagon, gut glucagon and insulin in human morbid obesity. Int J Obes. 1983; 7(6):529-38. View

15.

Thrivikraman K, Huot R, Plotsky P . Jugular vein catheterization for repeated blood sampling in the unrestrained conscious rat. Brain Res Brain Res Protoc. 2002; 10(2):84-94. DOI: 10.1016/s1385-299x(02)00185-x. View

16.

Valderas J, Irribarra V, Rubio L, Boza C, Escalona M, Liberona Y . Effects of sleeve gastrectomy and medical treatment for obesity on glucagon-like peptide 1 levels and glucose homeostasis in non-diabetic subjects. Obes Surg. 2011; 21(7):902-9. DOI: 10.1007/s11695-011-0375-4. View

17.

Diep T, Madsen A, Holst B, Kristiansen M, Wellner N, Hansen S . Dietary fat decreases intestinal levels of the anorectic lipids through a fat sensor. FASEB J. 2010; 25(2):765-74. DOI: 10.1096/fj.10-166595. View

18.

Basso N, Capoccia D, Rizzello M, Abbatini F, Mariani P, Maglio C . First-phase insulin secretion, insulin sensitivity, ghrelin, GLP-1, and PYY changes 72 h after sleeve gastrectomy in obese diabetic patients: the gastric hypothesis. Surg Endosc. 2011; 25(11):3540-50. DOI: 10.1007/s00464-011-1755-5. View

19.

Kashyap S, Daud S, Kelly K, Gastaldelli A, Win H, Brethauer S . Acute effects of gastric bypass versus gastric restrictive surgery on beta-cell function and insulinotropic hormones in severely obese patients with type 2 diabetes. Int J Obes (Lond). 2009; 34(3):462-71. PMC: 2838991. DOI: 10.1038/ijo.2009.254. View

20.

Melissas J, Koukouraki S, Askoxylakis J, Stathaki M, Daskalakis M, Perisinakis K . Sleeve gastrectomy: a restrictive procedure?. Obes Surg. 2007; 17(1):57-62. DOI: 10.1007/s11695-007-9006-5. View