Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-protected Gold Nanoparticles with Lipid Bilayers

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2013 Aug 7

PMID 23915118

Citations 61

Authors

Reid C Van Lehn

Prabhani U Atukorale

Randy P Carney

Yu-Sang Yang

Francesco Stellacci

Darrell J Irvine

Alfredo Alexander-Katz

Affiliations

Soon will be listed here.

Abstract

Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free energy calculations, experiments on unilamellar and multilamellar vesicles, and cell studies all support this hypothesis. Furthermore, we show that fusion is only favorable for AuNPs with core diameters below a critical size that depends on the monolayer composition.

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References

Carney R, Carney T, Mueller M, Stellacci F . Dynamic cellular uptake of mixed-monolayer protected nanoparticles. Biointerphases. 2012; 7(1-4):17. DOI: 10.1007/s13758-011-0017-3. View

Jackson A, Hu Y, Silva P, Stellacci F . From homoligand- to mixed-ligand- monolayer-protected metal nanoparticles: a scanning tunneling microscopy investigation. J Am Chem Soc. 2006; 128(34):11135-49. DOI: 10.1021/ja061545h. View

Nielsen C, Goulian M, Andersen O . Energetics of inclusion-induced bilayer deformations. Biophys J. 1998; 74(4):1966-83. PMC: 1299538. DOI: 10.1016/S0006-3495(98)77904-4. View

MacCallum J, Bennett W, Tieleman D . Partitioning of amino acid side chains into lipid bilayers: results from computer simulations and comparison to experiment. J Gen Physiol. 2007; 129(5):371-7. PMC: 2154372. DOI: 10.1085/jgp.200709745. View

Herce H, Garcia A . Molecular dynamics simulations suggest a mechanism for translocation of the HIV-1 TAT peptide across lipid membranes. Proc Natl Acad Sci U S A. 2007; 104(52):20805-10. PMC: 2409222. DOI: 10.1073/pnas.0706574105. View

Verma A, Uzun O, Hu Y, Hu Y, Han H, Watson N . Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. Nat Mater. 2008; 7(7):588-95. PMC: 2684029. DOI: 10.1038/nmat2202. View

White S, Wimley W . Membrane protein folding and stability: physical principles. Annu Rev Biophys Biomol Struct. 1999; 28:319-65. DOI: 10.1146/annurev.biophys.28.1.319. View

Strandberg E, Killian J . Snorkeling of lysine side chains in transmembrane helices: how easy can it get?. FEBS Lett. 2003; 544(1-3):69-73. DOI: 10.1016/s0014-5793(03)00475-7. View

Li Y, Li X, Li Z, Gao H . Surface-structure-regulated penetration of nanoparticles across a cell membrane. Nanoscale. 2012; 4(12):3768-75. DOI: 10.1039/c2nr30379e. View

10.

Von White 2nd G, Chen Y, Roder-Hanna J, Bothun G, Kitchens C . Structural and thermal analysis of lipid vesicles encapsulating hydrophobic gold nanoparticles. ACS Nano. 2012; 6(6):4678-85. DOI: 10.1021/nn2042016. View

11.

Leontiadou H, Mark A, Marrink S . Molecular dynamics simulations of hydrophilic pores in lipid bilayers. Biophys J. 2004; 86(4):2156-64. PMC: 1304067. DOI: 10.1016/S0006-3495(04)74275-7. View

12.

Ben-Tal N, Ben-Shaul A, Nicholls A, Honig B . Free-energy determinants of alpha-helix insertion into lipid bilayers. Biophys J. 1996; 70(4):1803-12. PMC: 1225150. DOI: 10.1016/S0006-3495(96)79744-8. View

13.

Shai Y . Mechanism of the binding, insertion and destabilization of phospholipid bilayer membranes by alpha-helical antimicrobial and cell non-selective membrane-lytic peptides. Biochim Biophys Acta. 1999; 1462(1-2):55-70. DOI: 10.1016/s0005-2736(99)00200-x. View

14.

Preiner J, Janovjak H, Rankl C, Knaus H, Cisneros D, Kedrov A . Free energy of membrane protein unfolding derived from single-molecule force measurements. Biophys J. 2007; 93(3):930-7. PMC: 1913163. DOI: 10.1529/biophysj.106.096982. View

15.

Nel A, Madler L, Velegol D, Xia T, Hoek E, Somasundaran P . Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater. 2009; 8(7):543-57. DOI: 10.1038/nmat2442. View

16.

Wang B, Zhang L, Bae S, Granick S . Nanoparticle-induced surface reconstruction of phospholipid membranes. Proc Natl Acad Sci U S A. 2008; 105(47):18171-5. PMC: 2587577. DOI: 10.1073/pnas.0807296105. View

17.

Carney R, Astier Y, Carney T, Voitchovsky K, Jacob Silva P, Stellacci F . Electrical method to quantify nanoparticle interaction with lipid bilayers. ACS Nano. 2012; 7(2):932-42. DOI: 10.1021/nn3036304. View

18.

Dorairaj S, Allen T . On the thermodynamic stability of a charged arginine side chain in a transmembrane helix. Proc Natl Acad Sci U S A. 2007; 104(12):4943-8. PMC: 1829244. DOI: 10.1073/pnas.0610470104. View

19.

Marks J, Placone J, Hristova K, Wimley W . Spontaneous membrane-translocating peptides by orthogonal high-throughput screening. J Am Chem Soc. 2011; 133(23):8995-9004. PMC: 3118567. DOI: 10.1021/ja2017416. View

20.

Lane J, Grest G . Spontaneous asymmetry of coated spherical nanoparticles in solution and at liquid-vapor interfaces. Phys Rev Lett. 2010; 104(23):235501. DOI: 10.1103/PhysRevLett.104.235501. View