PCR-mediated Site-directed Mutagenesis

Overview

Journal Cold Spring Harb Protoc

Specialties Biomedical Engineering
Pathology

Date 2013 Aug 3

PMID 23906918

Citations 20

Authors

Michael F Carey

Craig L Peterson

Stephen T Smale

Affiliations

Soon will be listed here.

Abstract

Unlike traditional site-directed mutagenesis, this protocol requires only a single PCR step using full plasmid amplification to generate point mutants. The method can introduce small mutations into promoter sites and is even better suited for introducing single or double mutations into proteins. It is elegant in its simplicity and can be applied quite easily in any laboratory using standard protein expression vectors and commercially available reagents.

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