Force Produced After Stretch in Sarcomeres and Half-sarcomeres Isolated from Skeletal Muscles

Overview

Journal Sci Rep

Specialty Science

Date 2013 Aug 1

PMID 23900500

Citations 20

Authors

Fabio C Minozzo

Bruno M Baroni

Jose A Correa

Marco A Vaz

Dilson E Rassier

Affiliations

Soon will be listed here.

Abstract

The goal of this study was to evaluate if isolated sarcomeres and half-sarcomeres produce a long-lasting increase in force after a stretch is imposed during activation. Single and half-sarcomeres were isolated from myofibrils using micro-needles, which were also used for force measurements. After full force development, both preparations were stretched by different magnitudes. The sarcomere length (SL) or half-sarcomere length variations (HSL) were extracted by measuring the initial and final distances from the Z-line to the adjacent Z-line or to a region externally adjacent to the M-line of the sarcomere, respectively. Half-sarcomeres generated approximately the same amount of isometric force (29.0 ± SD 15.5 nN·μm(-2)) as single sarcomeres (32.1 ± SD 15.3 nN·μm(-2)) when activated. In both cases, the steady-state forces after stretch were higher than the forces during isometric contractions at similar conditions. The results suggest that stretch-induced force enhancement is partly caused by proteins within the half-sarcomere.

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References

Ayittey P, Walker J, Rice J, de Tombe P . Glass microneedles for force measurements: a finite-element analysis model. Pflugers Arch. 2008; 457(6):1415-22. PMC: 2898131. DOI: 10.1007/s00424-008-0605-3. View

Pavlov I, Novinger R, Rassier D . Sarcomere dynamics in skeletal muscle myofibrils during isometric contractions. J Biomech. 2009; 42(16):2808-12. DOI: 10.1016/j.jbiomech.2009.08.011. View

Stienen G, Versteeg P, Papp Z, Elzinga G . Mechanical properties of skinned rabbit psoas and soleus muscle fibres during lengthening: effects of phosphate and Ca2+. J Physiol. 1992; 451:503-23. PMC: 1176173. DOI: 10.1113/jphysiol.1992.sp019176. View

Rassier D, Pavlov I . Force produced by isolated sarcomeres and half-sarcomeres after an imposed stretch. Am J Physiol Cell Physiol. 2011; 302(1):C240-8. DOI: 10.1152/ajpcell.00208.2011. View

Morgan D . New insights into the behavior of muscle during active lengthening. Biophys J. 1990; 57(2):209-21. PMC: 1280663. DOI: 10.1016/S0006-3495(90)82524-8. View

Minozzo F, Rassier D . Effects of blebbistatin and Ca2+ concentration on force produced during stretch of skeletal muscle fibers. Am J Physiol Cell Physiol. 2010; 299(5):C1127-35. DOI: 10.1152/ajpcell.00073.2010. View

Rassier D . Pre-power stroke cross bridges contribute to force during stretch of skeletal muscle myofibrils. Proc Biol Sci. 2008; 275(1651):2577-86. PMC: 2605803. DOI: 10.1098/rspb.2008.0719. View

Morgan D . An explanation for residual increased tension in striated muscle after stretch during contraction. Exp Physiol. 1994; 79(5):831-8. DOI: 10.1113/expphysiol.1994.sp003811. View

Bartoo M, Popov V, Fearn L, Pollack G . Active tension generation in isolated skeletal myofibrils. J Muscle Res Cell Motil. 1993; 14(5):498-510. DOI: 10.1007/BF00297212. View

10.

Colombini B, Nocella M, Bagni M, Griffiths P, Cecchi G . Is the cross-bridge stiffness proportional to tension during muscle fiber activation?. Biophys J. 2010; 98(11):2582-90. PMC: 2877350. DOI: 10.1016/j.bpj.2010.02.014. View

11.

Telley I, Stehle R, Ranatunga K, Pfitzer G, Stussi E, Denoth J . Dynamic behaviour of half-sarcomeres during and after stretch in activated rabbit psoas myofibrils: sarcomere asymmetry but no 'sarcomere popping'. J Physiol. 2006; 573(Pt 1):173-85. PMC: 1618761. DOI: 10.1113/jphysiol.2006.105809. View

12.

Pavlov I, Novinger R, Rassier D . The mechanical behavior of individual sarcomeres of myofibrils isolated from rabbit psoas muscle. Am J Physiol Cell Physiol. 2009; 297(5):C1211-9. DOI: 10.1152/ajpcell.00233.2009. View

13.

Leonard T, Herzog W . Regulation of muscle force in the absence of actin-myosin-based cross-bridge interaction. Am J Physiol Cell Physiol. 2010; 299(1):C14-20. DOI: 10.1152/ajpcell.00049.2010. View

14.

Pun C, Syed A, Rassier D . History-dependent properties of skeletal muscle myofibrils contracting along the ascending limb of the force-length relationship. Proc Biol Sci. 2009; 277(1680):475-84. PMC: 2842654. DOI: 10.1098/rspb.2009.1579. View

15.

Cornachione A, Rassier D . A non-cross-bridge, static tension is present in permeabilized skeletal muscle fibers after active force inhibition or actin extraction. Am J Physiol Cell Physiol. 2011; 302(3):C566-74. DOI: 10.1152/ajpcell.00355.2011. View

16.

Labeit D, Watanabe K, Witt C, Fujita H, Wu Y, Lahmers S . Calcium-dependent molecular spring elements in the giant protein titin. Proc Natl Acad Sci U S A. 2003; 100(23):13716-21. PMC: 263879. DOI: 10.1073/pnas.2235652100. View

17.

Julian F, Morgan D . The effect on tension of non-uniform distribution of length changes applied to frog muscle fibres. J Physiol. 1979; 293:379-92. PMC: 1280719. DOI: 10.1113/jphysiol.1979.sp012895. View

18.

Telley I, Denoth J, Stussi E, Pfitzer G, Stehle R . Half-sarcomere dynamics in myofibrils during activation and relaxation studied by tracking fluorescent markers. Biophys J. 2005; 90(2):514-30. PMC: 1367057. DOI: 10.1529/biophysj.105.070334. View

19.

Sbalzarini I, Koumoutsakos P . Feature point tracking and trajectory analysis for video imaging in cell biology. J Struct Biol. 2005; 151(2):182-95. DOI: 10.1016/j.jsb.2005.06.002. View

20.

Gordon A, HUXLEY A, Julian F . The variation in isometric tension with sarcomere length in vertebrate muscle fibres. J Physiol. 1966; 184(1):170-92. PMC: 1357553. DOI: 10.1113/jphysiol.1966.sp007909. View