Ratchet Nanofiltration of DNA

Overview

Journal Lab Chip

Publisher Royal Society of Chemistry

Specialties Biotechnology
Chemistry

Date 2013 Jul 31

PMID 23896739

Citations 2

Authors

Joel D P Thomas

Mark N Joswiak

Daniel W Olson

Sung-Gyu Park

Kevin D Dorfman

Affiliations

Soon will be listed here.

Abstract

The DNA nanofilter is a microfabricated electrophoretic separation device consisting of a periodic array of thin slits (circa 60 nm) separated by deeper wells (circa 320 nm). We demonstrate that this device can act as a tuneable, clog-free filter when operating in a low frequency, asymmetric field inversion mode. This filtration occurs by using asymmetric field inversion to achieve bi-directional migration of short (less than 1000 bp) DNA. Moreover, similar ratchet-type operation can improve separations when compared to a constant field separation in the same device. These modes of operation enhance the utility of the DNA nanofilter as a component of integrated lab-on-a-chip devices. The experimental data confirm theoretical predictions for the bidirectional transport of DNA in entropy-based separations.

Citing Articles

Entropic trap purification of long DNA.

Agrawal P, Bognar Z, Dorfman K Lab Chip. 2018; 18(6):955-964.

PMID: 29469139 PMC: 5849573. DOI: 10.1039/c7lc01355h.

Fluctuations of DNA mobility in nanofluidic entropic traps.

Wu L, Levy S Biomicrofluidics. 2014; 8(4):044103.

PMID: 25379088 PMC: 4189160. DOI: 10.1063/1.4887395.

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