Piceatannol Suppresses Endotoxin-induced Ocular Inflammation in Rats

Overview

Journal Int Immunopharmacol

Specialties Allergy & Immunology
Pharmacology

Date 2013 Jul 30

PMID 23892029

Citations 8

Authors

Nilesh M Kalariya

Mohammad Shoeb

Aramati B M Reddy

Rahul Sawhney

Kota V Ramana

Affiliations

Soon will be listed here.

Abstract

Anti-inflammatory effect of piceatannol, a naturally occurring polyphenol and a potent free radical scavenger, on ocular inflammation is not known. We examined the anti-inflammatory role of piceatannol in ocular inflammatory response due to endotoxin-induced uveitis (EIU) in rats. EIU was induced in Lewis rats by subcutaneous injection of lipopolysaccharide (LPS; 150 ug/rat). Piceatannol (30mg/kg body wt, i.p) was injected either 2h prior to or 1h post LPS induction. A significant increase in the number of infiltrating cells, total protein, and various cytokines and chemokines in AqH were observed in the EIU rat eyes as compared to control groups. However, pre- or post-treatment of piceatannol significantly blocked the LPS-induced changes. Further, piceatannol also suppressed the expression of cyclooxygenase-2 (Cox-2), inducible nitric oxide synthase (iNOS) and activation of NF-κB in the ciliary bodies as well as retina. Further, piceatannol also inhibited the expression of Cox-2, iNOS, and phosphorylation of NF-κB in primary human non-pigmented ciliary epithelial cells (HNPECs) treated with LPS. Similarly, piceatannol also diminished LPS-induced level of NO and prostaglandin E2 in HNPECs. Thus our results demonstrate an anti-inflammatory role of piceatannol in suppressing ocular inflammation induced by endotoxin in rats.

Citing Articles

Protective Effect of Piceatannol Against Acute Lung Injury Through Protecting the Integrity of Air-Blood Barrier and Modulating the TLR4/NF-κB Signaling Pathway Activation.

Peng L, Yuan M, Shi H, Li J, Song K, Huang J Front Pharmacol. 2020; 10:1613.

PMID: 32038265 PMC: 6988518. DOI: 10.3389/fphar.2019.01613.

Aldo-Keto Reductases: Multifunctional Proteins as Therapeutic Targets in Diabetes and Inflammatory Disease.

Chang K, Petrash J Adv Exp Med Biol. 2018; 1032:173-202.

PMID: 30362099 PMC: 8418822. DOI: 10.1007/978-3-319-98788-0_13.

Amelioration of Endotoxin-Induced Inflammatory Toxic Response by a Metal Chelator in Rat Eyes.

Shoeb M, Zhang M, Xiao T, Syed M, Ansari N Invest Ophthalmol Vis Sci. 2018; 59(1):31-38.

PMID: 29302691 PMC: 5754197. DOI: 10.1167/iovs.17-22172.

Expression Profile of Cationic Amino Acid Transporters in Rats with Endotoxin-Induced Uveitis.

Hsu Y, Chang S, Yang C, Lee Y, Kao T Mediators Inflamm. 2016; 2016:6586857.

PMID: 27413255 PMC: 4927963. DOI: 10.1155/2016/6586857.

Relative potency of tyrosol in the treatment of endotoxin-induced uveitis in rats.

Sato K, Mihara Y, Kanai K, Yamashita Y, Kimura Y, Itoh N J Vet Med Sci. 2016; 78(10):1631-1634.

PMID: 27350009 PMC: 5095636. DOI: 10.1292/jvms.16-0254.

References

Liu D, Kim D, Park J, Na H, Surh Y . Piceatannol inhibits phorbol ester-induced NF-kappa B activation and COX-2 expression in cultured human mammary epithelial cells. Nutr Cancer. 2010; 61(6):855-63. DOI: 10.1080/01635580903285080. View

Sijssens K, Rijkers G, Rothova A, Stilma J, de Boer J . Distinct cytokine patterns in the aqueous humor of children, adolescents and adults with uveitis. Ocul Immunol Inflamm. 2008; 16(5):211-6. DOI: 10.1080/09273940802409969. View

Rhayem Y, Therond P, Camont L, Couturier M, Beaudeux J, Legrand A . Chain-breaking activity of resveratrol and piceatannol in a linoleate micellar model. Chem Phys Lipids. 2008; 155(1):48-56. DOI: 10.1016/j.chemphyslip.2008.06.001. View

Kalariya N, Shoeb M, Reddy A, Zhang M, van Kuijk F, Ramana K . Prevention of endotoxin-induced uveitis in rats by plant sterol guggulsterone. Invest Ophthalmol Vis Sci. 2010; 51(10):5105-13. PMC: 2946978. DOI: 10.1167/iovs.09-4873. View

Morales P, Haza A . Selective apoptotic effects of piceatannol and myricetin in human cancer cells. J Appl Toxicol. 2011; 32(12):986-93. DOI: 10.1002/jat.1725. View

Choi K, Kim J, Song N, Son J, Hwang M, Byun S . Phosphoinositide 3-kinase is a novel target of piceatannol for inhibiting PDGF-BB-induced proliferation and migration in human aortic smooth muscle cells. Cardiovasc Res. 2009; 85(4):836-44. DOI: 10.1093/cvr/cvp359. View

Nguyen Q, Hatef E, Kayen B, Macahilig C, Ibrahim M, Wang J . A cross-sectional study of the current treatment patterns in noninfectious uveitis among specialists in the United States. Ophthalmology. 2010; 118(1):184-90. DOI: 10.1016/j.ophtha.2010.03.029. View

Son P, Park S, Na H, Jue D, Kim S, Surh Y . Piceatannol, a catechol-type polyphenol, inhibits phorbol ester-induced NF-{kappa}B activation and cyclooxygenase-2 expression in human breast epithelial cells: cysteine 179 of IKK{beta} as a potential target. Carcinogenesis. 2010; 31(8):1442-9. DOI: 10.1093/carcin/bgq099. View

Suzuki J, Manola A, Murakami Y, Morizane Y, Takeuchi K, Kayama M . Inhibitory effect of aminoimidazole carboxamide ribonucleotide (AICAR) on endotoxin-induced uveitis in rats. Invest Ophthalmol Vis Sci. 2011; 52(9):6565-71. DOI: 10.1167/iovs.11-7331. View

10.

Ashikawa K, Majumdar S, Banerjee S, Bharti A, Shishodia S, Aggarwal B . Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. J Immunol. 2002; 169(11):6490-7. DOI: 10.4049/jimmunol.169.11.6490. View

11.

Carnahan M, Goldstein D . Ocular complications of topical, peri-ocular, and systemic corticosteroids. Curr Opin Ophthalmol. 2001; 11(6):478-83. DOI: 10.1097/00055735-200012000-00016. View

12.

Youn J, Lee J, Na H, Kundu J, Surh Y . Resveratrol and piceatannol inhibit iNOS expression and NF-kappaB activation in dextran sulfate sodium-induced mouse colitis. Nutr Cancer. 2010; 61(6):847-54. DOI: 10.1080/01635580903285072. View

13.

de Vos A, Klaren V, Kijlstra A . Expression of multiple cytokines and IL-1RA in the uvea and retina during endotoxin-induced uveitis in the rat. Invest Ophthalmol Vis Sci. 1994; 35(11):3873-83. View

14.

Kim Y, Kwon H, Kim D, Cho H, Lee H, Jun J . Piceatannol, a stilbene present in grapes, attenuates dextran sulfate sodium-induced colitis. Int Immunopharmacol. 2008; 8(12):1695-702. DOI: 10.1016/j.intimp.2008.08.003. View

15.

Saxena A, Tammali R, Ramana K, Srivastava S . Aldose reductase inhibition prevents colon cancer growth by restoring phosphatase and tensin homolog through modulation of miR-21 and FOXO3a. Antioxid Redox Signal. 2012; 18(11):1249-62. PMC: 3584509. DOI: 10.1089/ars.2012.4643. View

16.

Wang J, Lu H, Hu X, Chen W, Xu Z, Li S . Nuclear factor translocation and acute anterior uveitis. Mol Vis. 2011; 17:170-6. PMC: 3025100. View

17.

Curnow S, Murray P . Inflammatory mediators of uveitis: cytokines and chemokines. Curr Opin Ophthalmol. 2006; 17(6):532-7. DOI: 10.1097/ICU.0b013e32801094b5. View

18.

Makena P, Chung K . Effects of various plant polyphenols on bladder carcinogen benzidine-induced mutagenicity. Food Chem Toxicol. 2007; 45(10):1899-909. DOI: 10.1016/j.fct.2007.04.007. View

19.

Keino H . [Therapeutic effect of the low molecular weight inhibitor of the NF-kappaB signaling pathway on experimental autoimmune uveoretinitis]. Nippon Ganka Gakkai Zasshi. 2010; 114(11):944-54. View

20.

Richard N, Porath D, Radspieler A, Schwager J . Effects of resveratrol, piceatannol, tri-acetoxystilbene, and genistein on the inflammatory response of human peripheral blood leukocytes. Mol Nutr Food Res. 2005; 49(5):431-42. DOI: 10.1002/mnfr.200400099. View