Phenotypic Characterisation of the Cellular Immune Infiltrate in Placentas of Cattle Following Experimental Inoculation with Neospora Caninum in Late Gestation

Overview

Journal Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2013 Jul 24

PMID 23876124

Citations 7

Authors

German J Canton

Frank Katzer

Julio Benavides-Silvan

Stephen W Maley

Javier Palarea-Albaladejo

Yvonne Pang

Sionagh Smith

Paul M Bartley

Mara Rocchi

Elisabeth A Innes

Francesca Chianini

Affiliations

Soon will be listed here.

Abstract

Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation.

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