Effect of Ocimum Basilicum Extract on Cadmium-induced Testicular Histomorphometric and Immunohistochemical Alterations in Albino Rats

Overview

Journal Anat Cell Biol

Specialty Anatomy & Histology

Date 2013 Jul 23

PMID 23869259

Citations 10

Authors

Saber A Sakr

Hanna Z Nooh

Affiliations

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Abstract

The present study examined the efficacy of Ocimum basilicum (basil) extract, a natural herb, with antioxidant properties, against testicular toxicity induced by cadmium (Cd), which is one of the most important toxic heavy metals. The intoxicated rats showed significant alterations in the testicular tissue including decreased seminiferous epithelium height and changes in the arrangement of spermatogenic layers. Hypospermatogensis with cytoplasmic vacuolization and pyknotic nuclei were observed. Intertubular hemorrahage and absence of spermatozoa were noted. Decreased cell proliferation was reflected by a decrease in Ki-67 expression, whereas the increase in apoptotic rate was associated with a decrease in the Bcl/Bax ratio. Concomitant treatment with aqueous basil extract led to an improvement in histological, morphometrical and immunohistochemical changes induced by Cd. The beneficial effects of basil extract could be attributed to its antioxidant properties.

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