» Articles » PMID: 23869045

Proteomic Analysis of Eggs from Mytilus Edulis Females Differing in Mitochondrial DNA Transmission Mode

Overview
Date 2013 Jul 23
PMID 23869045
Citations 9
Authors
Affiliations
Soon will be listed here.
Abstract

Many bivalves have an unusual mechanism of mitochondrial DNA (mtDNA) inheritance called doubly uniparental inheritance (DUI) in which distinctly different genomes are inherited through the female (F genome) and male (M genome) lineages. In fertilized eggs that will develop into male embryos, the sperm mitochondria remain in an aggregation, which is believed to be delivered to the primordial germ cells and passed to the next generation through the sperm. In fertilized eggs that will develop into female embryos, the sperm mitochondria are dispersed throughout the developing embryo and make little if any contribution to the next generation. The frequency of embryos with the aggregated or dispersed mitochondrial type varies among females. Previous models of DUI have predicted that maternal nuclear factors cause molecular differences among unfertilized eggs from females producing embryos with predominantly dispersed or aggregated mitochondria. We test this hypothesis using females of each of the two types from a natural population. We have found small, yet detectable, differences of the predicted type at the proteome level. We also provide evidence that eggs of females giving the dispersed pattern have consistently lower expression for different proteasome subunits than eggs of females giving the aggregated pattern. These results, combined with those of an earlier study in which we used hatchery lines of Mytilus, and with a transcriptomic study in a clam that has the DUI system of mtDNA transmission, reinforce the hypothesis that the ubiquitin-proteasome system plays a key role in the mechanism of DUI and sex determination in bivalves. We also report that eggs of females giving the dispersed pattern have higher expression for arginine kinase and enolase, enzymes involved in energy production, whereas ferritin, which is involved in iron homeostasis, has lower expression. We discuss these results in the context of genetic models for DUI and suggest experimental methods for further understanding the role of these proteins in DUI.

Citing Articles

Molluscan mitochondrial genomes break the rules.

Ghiselli F, Gomes-Dos-Santos A, Adema C, Lopes-Lima M, Sharbrough J, Boore J Philos Trans R Soc Lond B Biol Sci. 2021; 376(1825):20200159.

PMID: 33813887 PMC: 8059616. DOI: 10.1098/rstb.2020.0159.


RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species.

Romero M, Perez-Figueroa A, Carrera M, Swanson W, Skibinski D, Diz A J Proteomics. 2018; 192:169-187.

PMID: 30189323 PMC: 6309513. DOI: 10.1016/j.jprot.2018.08.020.


Deciphering the Link between Doubly Uniparental Inheritance of mtDNA and Sex Determination in Bivalves: Clues from Comparative Transcriptomics.

Capt C, Renaut S, Ghiselli F, Milani L, Johnson N, Sietman B Genome Biol Evol. 2018; 10(2):577-590.

PMID: 29360964 PMC: 5800059. DOI: 10.1093/gbe/evy019.


Structure-Related Differences between Cytochrome Oxidase I Proteins in a Stable Heteroplasmic Mitochondrial System.

Skibinski D, Ghiselli F, Diz A, Milani L, Mullins J Genome Biol Evol. 2017; 9(12):3265-3281.

PMID: 29149282 PMC: 5726481. DOI: 10.1093/gbe/evx235.


SmithRNAs: Could Mitochondria "Bend" Nuclear Regulation?.

Pozzi A, Plazzi F, Milani L, Ghiselli F, Passamonti M Mol Biol Evol. 2017; 34(8):1960-1973.

PMID: 28444389 PMC: 5850712. DOI: 10.1093/molbev/msx140.


References
1.
Song J, Wessel G . How to make an egg: transcriptional regulation in oocytes. Differentiation. 2005; 73(1):1-17. DOI: 10.1111/j.1432-0436.2005.07301005.x. View

2.
Diz A, Martinez-Fernandez M, Rolan-Alvarez E . Proteomics in evolutionary ecology: linking the genotype with the phenotype. Mol Ecol. 2012; 21(5):1060-80. DOI: 10.1111/j.1365-294X.2011.05426.x. View

3.
Cao S, Guo X, Zhou Z, Sha J . Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice. Mol Reprod Dev. 2012; 79(6):413-22. DOI: 10.1002/mrd.22044. View

4.
Skibinski D, Gallagher C, Beynon C . Mitochondrial DNA inheritance. Nature. 1994; 368(6474):817-8. DOI: 10.1038/368817b0. View

5.
Obata M, Shimizu M, Sano N, Komaru A . Maternal inheritance of mitochondrial DNA (mtDNA) in the Pacific oyster (Crassostrea gigas): a preliminary study using mtDNA sequence analysis with evidence of random distribution of MitoTracker-stained sperm mitochondria in fertilized eggs. Zoolog Sci. 2008; 25(3):248-54. DOI: 10.2108/zsj.25.248. View