Assessment of Hemodynamics in a Rat Model of Liver Cirrhosis with Precancerous Lesions Using Multislice Spiral CT Perfusion Imaging

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2013 Jul 19

PMID 23865067

Citations 6

Authors

Guolin Ma

Rongjie Bai

Huijie Jiang

Xuejia Hao

Zaisheng Ling

Kefeng Li

Affiliations

Soon will be listed here.

Abstract

Rationale And Objectives: To develop an optimal scanning protocol for multislice spiral CT perfusion (CTP) imaging to evaluate hemodynamic changes in liver cirrhosis with diethylnitrosamine- (DEN-) induced precancerous lesions.

Materials And Methods: Male Wistar rats were randomly divided into the control group (n = 80) and the precancerous liver cirrhosis group (n = 40). The control group received saline injection and the liver cirrhosis group received 50 mg/kg DEN i.p. twice a week for 12 weeks. All animals underwent plain CT scanning, CTP, and contrast-enhanced CT scanning. Scanning parameters were optimized by adjusting the diatrizoate concentration, the flow rate, and the delivery time. The hemodynamics of both groups was further compared using optimized multislice spiral CTP imaging.

Results: High-quality CTP images were obtained with following parameters: 150 kV; 150 mAs; 5 mm thickness, 5 mm interval; pitch, 1; matrix, 512 × 512; and FOV, 9.6 cm. Compared to the control group, the liver cirrhosis group had a significantly increased value of the hepatic arterial fraction and the hepatic artery perfusion (P < 0.05) but significantly decreased hepatic portal perfusion and mean transit time (P < 0.05).

Conclusion: Multislice spiral CTP imaging can be used to evaluate the hemodynamic changes in the rat model of liver cirrhosis with precancerous lesions.

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References

Heindryckx F, Colle I, Van Vlierberghe H . Experimental mouse models for hepatocellular carcinoma research. Int J Exp Pathol. 2009; 90(4):367-86. PMC: 2741148. DOI: 10.1111/j.1365-2613.2009.00656.x. View

Bernstine H, Braun M, Yefremov N, Lamash Y, Carmi R, Stern D . FDG PET/CT early dynamic blood flow and late standardized uptake value determination in hepatocellular carcinoma. Radiology. 2011; 260(2):503-10. DOI: 10.1148/radiol.11102350. View

Goh V, Sarker D, Osmany S, Cook G . Functional imaging techniques in hepatocellular carcinoma. Eur J Nucl Med Mol Imaging. 2012; 39(6):1070-9. DOI: 10.1007/s00259-012-2096-x. View

Guo D, Qiu T, Bian J, Liu S, Wang C . Detection and characterization of hepatocellular carcinoma in rats with liver cirrhosis: diagnostic value of combined use of MR positive and negative contrast agents. Hepatobiliary Pancreat Dis Int. 2009; 8(1):65-70. View

Lai K, Shang S, Lohia N, Booth G, Masse D, Fausto N . Extracellular matrix dynamics in hepatocarcinogenesis: a comparative proteomics study of PDGFC transgenic and Pten null mouse models. PLoS Genet. 2011; 7(6):e1002147. PMC: 3121762. DOI: 10.1371/journal.pgen.1002147. View

Li X, Zhou X, Guan Y, Wang Y . Magnetic resonance imaging of hepatocellular carcinoma induced by diethylnitrosamine in Sprague-Dawley rats. Hepatobiliary Pancreat Dis Int. 2005; 4(3):427-32. View

Tsushima Y, Blomley J, Kusano S, Endo K . The portal component of hepatic perfusion measured by dynamic CT: an indicator of hepatic parenchymal damage. Dig Dis Sci. 1999; 44(8):1632-8. DOI: 10.1023/a:1026679430553. View

Jiang H, Zhang Z, Shen B, Wan Y, Guo H, Shu S . Functional CT for assessment of early vascular physiology in liver tumors. Hepatobiliary Pancreat Dis Int. 2008; 7(5):497-502. View

Park D, Shin J, Park S, Seo J, Li L, Jang J . Diethylnitrosamine (DEN) induces irreversible hepatocellular carcinogenesis through overexpression of G1/S-phase regulatory proteins in rat. Toxicol Lett. 2009; 191(2-3):321-6. DOI: 10.1016/j.toxlet.2009.09.016. View

10.

Fournier L, Cuenod C, de Bazelaire C, Siauve N, Rosty C, Tran P . Early modifications of hepatic perfusion measured by functional CT in a rat model of hepatocellular carcinoma using a blood pool contrast agent. Eur Radiol. 2004; 14(11):2125-33. DOI: 10.1007/s00330-004-2339-8. View

11.

Choi S, Chung J, Kim H, Kim H, Baek J, Park C . The role of perfusion CT as a follow-up modality after transcatheter arterial chemoembolization: an experimental study in a rabbit model. Invest Radiol. 2010; 45(7):427-36. DOI: 10.1097/RLI.0b013e3181e07516. View

12.

Deng J, Jin N, Yin X, Yang G, Zhang Z, Omary R . Quantitative multiparametric PROPELLER MRI of diethylnitrosamine-induced hepatocarcinogenesis in wister rat model. J Magn Reson Imaging. 2010; 31(5):1242-51. PMC: 2905671. DOI: 10.1002/jmri.22138. View

13.

Gaspar T, Dvir D, Peled N . The role of 16-slice computed tomography angiography in the diagnosis of coronary artery disease: large sample analysis. Isr Med Assoc J. 2005; 7(7):424-7. View

14.

Koen H, Pugh T, Goldfarb S . Centrilobular distribution of diethylnitrosamine-induced hepatocellular foci in the mouse. Lab Invest. 1983; 49(1):78-81. View

15.

Conway J, Popp J, Thurman R . Microcirculation of hepatic nodules from diethylnitrosamine-treated rats. Cancer Res. 1985; 45(8):3620-5. View

16.

Kan Z, Phongkitkarun S, Kobayashi S, Tang Y, Ellis L, Lee T . Functional CT for quantifying tumor perfusion in antiangiogenic therapy in a rat model. Radiology. 2005; 237(1):151-8. DOI: 10.1148/radiol.2363041293. View

17.

Chuang S, Kuo M, Hsu C, Chen C, Lin J, Lai G . Curcumin-containing diet inhibits diethylnitrosamine-induced murine hepatocarcinogenesis. Carcinogenesis. 2000; 21(2):331-5. DOI: 10.1093/carcin/21.2.331. View

18.

Ippolito D, Capraro C, Casiraghi A, Cestari C, Sironi S . Quantitative assessment of tumour associated neovascularisation in patients with liver cirrhosis and hepatocellular carcinoma: role of dynamic-CT perfusion imaging. Eur Radiol. 2011; 22(4):803-11. DOI: 10.1007/s00330-011-2307-z. View

19.

Schneider C, Teufel A, Yevsa T, Staib F, Hohmeyer A, Walenda G . Adaptive immunity suppresses formation and progression of diethylnitrosamine-induced liver cancer. Gut. 2012; 61(12):1733-43. PMC: 4533880. DOI: 10.1136/gutjnl-2011-301116. View

20.

Gaiani S, Bolondi L, Li Bassi S, Zironi G, Siringo S, Barbara L . Prevalence of spontaneous hepatofugal portal flow in liver cirrhosis. Clinical and endoscopic correlation in 228 patients. Gastroenterology. 1991; 100(1):160-7. DOI: 10.1016/0016-5085(91)90596-d. View