Saliva As a Source of Anti-Toxoplasma Gondii IgG for Enzyme Immunoassay in Human Samples

Overview

Journal Clin Microbiol Infect

Publisher Elsevier

Specialty Infectious Diseases

Date 2013 Jul 16

PMID 23848317

Citations 8

Authors

B F C Sampaio

M S Macre

L R Meireles

H F Andrade Jr

Affiliations

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Abstract

Toxoplasmosis, a highly prevalent disease, is mainly diagnosed by serology. Incidence studies could be feasible in children, but ethical concerns restrict blood sampling in this group. Saliva contains small amounts of crevicular fluid IgG. Dot-ELISA and a protein A IgG capture immunoassay were standardized for anti-Toxoplasma gondii IgG in paired saliva and serum samples from 20 adult volunteers. A frequency of toxoplasmosis of 19% (95% CI 12-28) was found in 100 saliva samples from university graduates using both assays. Toxoplasmosis immunoassays using saliva IgG are a promising tool for the investigation of the epidemiology of this disease in children and other vulnerable groups.

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