Influence of the Carboxymethyl Chitosan Anti-adhesion Solution on the TGF-β1 in a Postoperative Peritoneal Adhesion Rat

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2013 Jul 4

PMID 23820936

Citations 4

Authors

Zengjuan Zheng

Weifen Zhang

Weiqing Sun

Xiaojian Li

Jinghua Duan

Juanjuan Cui

Zhanqin Feng

Heidi M Mansour

Affiliations

Soon will be listed here.

Abstract

The aim of this paper was to investigate the effect of carboxymethyl chitosan anti-adhesion solution on prevention of postsurgical adhesion. Forty adult male Wistar rats were randomly divided into three groups: 0.9% normal saline solution (group A), hyaluronic acid gels (group B) and carboxymethyl chitosan anti-adhesion solution (group C). The animals were treated with normal saline, hyaluronic acid gels or carboxymethyl chitosan anti-adhesion solution at the time of surgery. After 2 or 3 weeks, the degree of adhesions and histological effects were determined. The adhesions in groups B and C were significantly decreased, and the levels of TGF-β1 and hydroxyproline in group C were significantly lower than that in group A (P < 0.05). The histopathology in group C showed fewer inflammatory cells and fibroblasts. Carboxymethyl chitosan anti-adhesion solution can effectively prevent postoperative adhesion which is a promising drug delivery system in the context of postsurgical anti-adhesion.

Citing Articles

Towards Polycaprolactone-Based Scaffolds for Alveolar Bone Tissue Engineering: A Biomimetic Approach in a 3D Printing Technique.

Stafin K, Sliwa P, Piatkowski M Int J Mol Sci. 2023; 24(22).

PMID: 38003368 PMC: 10671727. DOI: 10.3390/ijms242216180.

Advances in Chitosan-Based Nanoparticles for Drug Delivery.

Mikusova V, Mikus P Int J Mol Sci. 2021; 22(17).

PMID: 34502560 PMC: 8431817. DOI: 10.3390/ijms22179652.

Antifibrotic Effect of Polysaccharide-Resveratrol-Impregnated Dual-Layer Carboxymethyl Cellulose-Based Sponge for The Prevention of Epidural Fibrosis after Laminectomy.

Chen H, Lin T, Chiang C, Wey S, Lin F, Yang K Polymers (Basel). 2021; 13(13).

PMID: 34209540 PMC: 8271895. DOI: 10.3390/polym13132129.

[Study on the prevention of postoperative intraperitoneal adhesion of rat with PLGA/PEG electrospun polymer membrane].

Chen Z, Zhao Y, He T, Chen K, Jiang L, Zhang J Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018; 31(8):999-1005.

PMID: 29806441 PMC: 8458602. DOI: 10.7507/1002-1892.201703083.

References

Sahin M, Cakir M, Avsar F, Tekin A, Kucukkartallar T, Akoz M . The effects of anti-adhesion materials in preventing postoperative adhesion in abdominal cavity (anti-adhesion materials for postoperative adhesions). Inflammation. 2007; 30(6):244-9. DOI: 10.1007/s10753-007-9043-1. View

Di Filippo C, Petronella P, Freda F, Scorzelli M, Ferretti M, Canonico S . Involvement of the ubiquitin-proteasome system in the formation of experimental postsurgical peritoneal adhesions. Mediators Inflamm. 2012; 2012:194723. PMC: 3306991. DOI: 10.1155/2012/194723. View

Jiang D, Xu C, Li Z, Zhang Y, Han F, Jiang Z . Protective action of hepatocyte growth factor on transforming growth factor beta-1-induced alpha-smooth muscle actin and extracellular matrix in cultured human peritoneal fibroblasts. Med Sci Monit. 2010; 16(8):BR250-4. View

Harris E, MORGAN R, Rodeheaver G . Analysis of the kinetics of peritoneal adhesion formation in the rat and evaluation of potential antiadhesive agents. Surgery. 1995; 117(6):663-9. DOI: 10.1016/s0039-6060(95)80010-7. View

Ryan C, Sax H . Evaluation of a carboxymethylcellulose sponge for prevention of postoperative adhesions. Am J Surg. 1995; 169(1):154-9; discussion 159-60. DOI: 10.1016/s0002-9610(99)80125-1. View

Lou W, Zhang H, Ma J, Zhang D, Liu C, Wang S . In vivo evaluation of in situ polysaccharide based hydrogel for prevention of postoperative adhesion. Carbohydr Polym. 2012; 90(2):1024-31. DOI: 10.1016/j.carbpol.2012.06.037. View

Lopes J, Dallan L, Moreira L, Campana Filho S, Gutierrez P, Lisboa L . Synergism between keratinocyte growth factor and carboxymethyl chitosan reduces pericardial adhesions. Ann Thorac Surg. 2010; 90(2):566-72. DOI: 10.1016/j.athoracsur.2010.03.086. View

Francesko A, Tzanov T . Chitin, chitosan and derivatives for wound healing and tissue engineering. Adv Biochem Eng Biotechnol. 2010; 125:1-27. DOI: 10.1007/10_2010_93. View

Kutlay J, Ozer Y, Isik B, Kargici H . Comparative effectiveness of several agents for preventing postoperative adhesions. World J Surg. 2004; 28(7):662-5. DOI: 10.1007/s00268-004-6825-6. View

10.

Schneider A, Bennek J, Olsen K, Weiss J, Schmidt W, Rolle U . Experimental study evaluating the effect of a barrier method on postoperative intraabdominal adhesions. Dig Dis Sci. 2006; 51(3):566-70. DOI: 10.1007/s10620-006-3171-6. View

11.

Yang B, Gong C, Zhao X, Zhou S, Li Z, Qi X . Preventing postoperative abdominal adhesions in a rat model with PEG-PCL-PEG hydrogel. Int J Nanomedicine. 2012; 7:547-57. PMC: 3277435. DOI: 10.2147/IJN.S26141. View

12.

Dong W, Han B, Feng Y, Song F, Chang J, Jiang H . Pharmacokinetics and biodegradation mechanisms of a versatile carboxymethyl derivative of chitosan in rats: in vivo and in vitro evaluation. Biomacromolecules. 2010; 11(6):1527-33. DOI: 10.1021/bm100158p. View

13.

Nair S, Bhat I, Aurora A . Role of proteolytic enzyme in the prevention of postoperative intraperitoneal adhesions. Arch Surg. 1974; 108(6):849-53. DOI: 10.1001/archsurg.1974.01350300081019. View

14.

Liuyun J, Yubao L, Chengdong X . A novel composite membrane of chitosan-carboxymethyl cellulose polyelectrolyte complex membrane filled with nano-hydroxyapatite I. Preparation and properties. J Mater Sci Mater Med. 2009; 20(8):1645-52. DOI: 10.1007/s10856-009-3720-6. View

15.

Mulier K, Nguyen A, Delaney J, Marquez S . Comparison of Permacol™ and Strattice™ for the repair of abdominal wall defects. Hernia. 2011; 15(3):315-9. DOI: 10.1007/s10029-010-0777-6. View

16.

Attard J, MacLean A . Adhesive small bowel obstruction: epidemiology, biology and prevention. Can J Surg. 2007; 50(4):291-300. PMC: 2386166. View

17.

Zhou J, Liwski R, Elson C, Lee T . Reduction in postsurgical adhesion formation after cardiac surgery in a rabbit model using N,O-carboxymethyl chitosan to block cell adherence. J Thorac Cardiovasc Surg. 2008; 135(4):777-83. DOI: 10.1016/j.jtcvs.2007.09.033. View

18.

Wei C, Hou C, Gu Q, Jiang L, Zhu B, Sheng A . A thermosensitive chitosan-based hydrogel barrier for post-operative adhesions' prevention. Biomaterials. 2009; 30(29):5534-40. DOI: 10.1016/j.biomaterials.2009.05.084. View

19.

Chang J, Thunder R, Most D, Longaker M, Lineaweaver W . Studies in flexor tendon wound healing: neutralizing antibody to TGF-beta1 increases postoperative range of motion. Plast Reconstr Surg. 2000; 105(1):148-55. DOI: 10.1097/00006534-200001000-00025. View

20.

Lauder C, Garcea G, Strickland A, Maddern G . Use of a modified chitosan-dextran gel to prevent peritoneal adhesions in a rat model. J Surg Res. 2010; 171(2):877-82. DOI: 10.1016/j.jss.2010.06.028. View