Evaluation of Bone Healing in Canine Tibial Defects Filled with Cortical Autograft, Commercial-DBM, Calf Fetal DBM, Omentum and Omentum-calf Fetal DBM

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2013 Jul 4

PMID 23820162

Citations 7

Authors

Amin Bigham-Sadegh

Iraj Karimi

Mahsa Alebouye

Zahra Shafie-Sarvestani

Ahmad Oryan

Affiliations

Soon will be listed here.

Abstract

The present study was conducted to compare the effects of xenogenic bovine fetal demineralized bone matrix (DBM), commercial DBM, omentum, omentum-calf fetal DBM, cortical autograft and xenogenic cartilage powder on the healing of tibial defects in a dog model to determine the best material for bone healing. Seven male adult mongrel dogs, weighing 26.2 ± 2.5 kg, were used in this study. Seven holes with a diameter of 4-mm were created and then filled with several biomaterials. Radiographs were taken postoperatively on day 1 and weeks 2, 4, 6, 8. The operated tibias were removed on the 56th postoperative day and histopathologically evaluated. On postoperative days 14, 42 and 56, the lesions of the control group were significantly inferior to those in the other group (p < 0.05). On the 28th postoperative day, the autograft group was significantly superior to the control and omentum groups (p < 0.05). Moreover, calf fetal DBM was significantly superior to the control group. There was no significant difference between the histopathological sections of all groups. Overall, the omentum and omentum-DBM groups were superior to the control group, but inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal cartilage groups.

Citing Articles

Histopathological and radiographical evaluation of caprine demineralized bone matrix in a critical ulnar defect in a rabbit model.

Alimi O, Abubakar A, Yakubu A, Shehu S, Abdulkadir S J Orthop Surg Res. 2022; 17(1):561.

PMID: 36550518 PMC: 9783744. DOI: 10.1186/s13018-022-03454-1.

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit.

Anaraki N, Beyraghi A, Raisi A, Davoodi F, Farjanikish G, Sadegh A J Orthop Surg Res. 2021; 16(1):362.

PMID: 34098995 PMC: 8183070. DOI: 10.1186/s13018-021-02498-z.

Pedicled vascularized bone grafts compared with xenografts in the treatment of scaphoid nonunion.

Matic S, Vuckovic C, Lesic A, Glisovic Jovanovic I, Polojac D, Ducic S Int Orthop. 2020; 45(4):1017-1023.

PMID: 32995916 DOI: 10.1007/s00264-020-04828-y.

Allogenic Bone Graft Enriched by Periosteal Stem Cell and Growth Factors for Osteogenesis in Critical Size Bone Defect in Rabbit Model: Histopathological and Radiological Evaluation.

Hassibi H, Farsinejad A, Dabiri S, Voosough D, Mortezaeizadeh A, Kheirandish R Iran J Pathol. 2020; 15(3):205-216.

PMID: 32754216 PMC: 7354065. DOI: 10.30699/ijp.2020.101715.2013.

Native Bovine Hydroxyapatite Powder, Demineralised Bone Matrix Powder, and Purified Bone Collagen Membranes Are Efficient in Repair of Critical-Sized Rat Calvarial Defects.

Veremeev A, Bolgarin R, Nesterenko V, Andreev-Andrievskiy A, Kutikhin A Materials (Basel). 2020; 13(15).

PMID: 32751921 PMC: 7436118. DOI: 10.3390/ma13153393.

References

Reddi A . Bone morphogenetic proteins, bone marrow stromal cells, and mesenchymal stem cells. Maureen Owen revisited. Clin Orthop Relat Res. 1995; (313):115-9. View

Tanaka T, Fujii K, Ohta M, Soshi S, Kitamura A, MUROTA K . Use of a guanidine extract of demineralized bone in the treatment of osteochondral defects of articular cartilage. J Orthop Res. 1995; 13(3):464-9. DOI: 10.1002/jor.1100130322. View

Khan S, Cammisa Jr F, Sandhu H, Diwan A, Girardi F, Lane J . The biology of bone grafting. J Am Acad Orthop Surg. 2005; 13(1):77-86. View

Guizzardi S, Di Silvestre M, Scandroglio R, Ruggeri A, SAVINI R . Implants of heterologous demineralized bone matrix for induction of posterior spinal fusion in rats. Spine (Phila Pa 1976). 1992; 17(6):701-7. DOI: 10.1097/00007632-199206000-00010. View

Alexander J . Bone grafting. Vet Clin North Am Small Anim Pract. 1987; 17(4):811-9. DOI: 10.1016/s0195-5616(87)50078-x. View

Takada T, Kamei Y, Iwata T, Yokoi T, Torii S . Effect of omental lipid fraction on enhancement of skin flap survival. Ann Plast Surg. 1998; 41(1):70-4. DOI: 10.1097/00000637-199807000-00013. View

Urist M, Sato K, Brownell A, Malinin T, LIETZE A, Huo Y . Human bone morphogenetic protein (hBMP). Proc Soc Exp Biol Med. 1983; 173(2):194-9. DOI: 10.3181/00379727-173-41630. View

Kirker-Head C . Recombinant bone morphogenetic proteins: novel substances for enhancing bone healing. Vet Surg. 1995; 24(5):408-19. DOI: 10.1111/j.1532-950x.1995.tb01352.x. View

Bostrom M, Lane J, Berberian W, Missri A, Tomin E, Weiland A . Immunolocalization and expression of bone morphogenetic proteins 2 and 4 in fracture healing. J Orthop Res. 1995; 13(3):357-67. DOI: 10.1002/jor.1100130309. View

10.

Cook S, Baffes G, Wolfe M, Sampath T, Rueger D . Recombinant human bone morphogenetic protein-7 induces healing in a canine long-bone segmental defect model. Clin Orthop Relat Res. 1994; (301):302-12. View

11.

Inoue K, Ohgushi H, Yoshikawa T, Okumura M, Sempuku T, Tamai S . The effect of aging on bone formation in porous hydroxyapatite: biochemical and histological analysis. J Bone Miner Res. 1997; 12(6):989-94. DOI: 10.1359/jbmr.1997.12.6.989. View

12.

Urist M, Strates B . Bone formation in implants of partially and wholly demineralized bone matrix. Including observations on acetone-fixed intra and extracellular proteins. Clin Orthop Relat Res. 1970; 71:271-8. View

13.

Friedlaender G . Bone grafts. The basic science rationale for clinical applications. J Bone Joint Surg Am. 1987; 69(5):786-90. View

14.

Bauer T, Muschler G . Bone graft materials. An overview of the basic science. Clin Orthop Relat Res. 2000; (371):10-27. View

15.

Jin D . [Bone matrix gelatin. Clinical application in 38 cases]. Zhonghua Wai Ke Za Zhi. 1991; 29(5):312-4, 335. View

16.

Gordon S, Warren R . Autogenous Diced Cartilage Transplants to Bone: An Experimental Study. Ann Surg. 1947; 125(2):237-40. PMC: 1803170. DOI: 10.1097/00000658-194702000-00009. View

17.

Reddi A, Huggins C . Biochemical sequences in the transformation of normal fibroblasts in adolescent rats. Proc Natl Acad Sci U S A. 1972; 69(6):1601-5. PMC: 426757. DOI: 10.1073/pnas.69.6.1601. View

18.

Martin Jr G, Boden S, Titus L, Scarborough N . New formulations of demineralized bone matrix as a more effective graft alternative in experimental posterolateral lumbar spine arthrodesis. Spine (Phila Pa 1976). 1999; 24(7):637-45. DOI: 10.1097/00007632-199904010-00005. View

19.

Urist M, MIKULSKI A, LIETZE A . Solubilized and insolubilized bone morphogenetic protein. Proc Natl Acad Sci U S A. 1979; 76(4):1828-32. PMC: 383485. DOI: 10.1073/pnas.76.4.1828. View

20.

Diaz-Flores L, Gutierrez R, Lopez-Alonso A, Gonzalez R, Varela H . Pericytes as a supplementary source of osteoblasts in periosteal osteogenesis. Clin Orthop Relat Res. 1992; (275):280-6. View