Calcium Release Modulated by Inositol Trisphosphate in Ruptured Fibers from Frog Skeletal Muscle

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1990 May 1

PMID 2381765

Citations 8

Authors

C Rojas

E Jaimovich

Affiliations

Soon will be listed here.

Abstract

To investigate the effect of inositol 1,4,5-trisphosphate on calcium release, we used fiber bundles of frog sartorius muscle mechanically permeabilized by a scratching procedure, and we detected increments in calcium concentration by measuring aqueorin light signals. Submicromolar concentrations of inositol 1,4,5-trisphosphate induced fast calcium-release signals, with a half time to peak of 60 ms or less. Similar responses were elicited by caffeine. The calcium-release signal induced by inositol 1,4,5-trisphosphate occurred at pCa values of 7 or lower, and the dose-response curve depended on the ionic composition of the incubation solution. Lower inositol 1,4,5-trisphosphate concentrations were needed to induce release when incubation solutions of ionic composition expected to depolarize the transverse tubule membrane were used. Inositol 1,4,5-trisphosphate was more effective than inositol 1,3,4-trisphosphate, inositol 1,4,5,6-tetrakisphosphate, and inositol 1,4-bisphosphate. The effect of inositol 1,4,5-trisphosphate was synergistic with that of caffeine, and was not inhibited by heparin. These results, by showing directly that at resting calcium levels inositol 1,4,5-trisphosphate elicited calcium release, are consistent with a role for inositol 1,4,5-trisphosphate as a chemical modulator in excitation/contraction coupling in skeletal muscle.

Citing Articles

Investigation of the effect of inositol trisphosphate in skinned skeletal muscle fibres with functional excitation-contraction coupling.

Posterino G, Lamb G J Muscle Res Cell Motil. 1998; 19(1):67-74.

PMID: 9477378 DOI: 10.1007/BF03257391.

Inositol 1,4,5-trisphosphate-induced Ca2+ release is regulated by cytosolic Ca2+ in intact skeletal muscle.

Lopez J, Terzic A Pflugers Arch. 1996; 432(5):782-90.

PMID: 8772127 DOI: 10.1007/s004240050199.

Effects of heparin on excitation-contraction coupling in skeletal muscle toad and rat.

Lamb G, Posterino G, Stephenson D J Physiol. 1994; 474(2):319-29.

PMID: 7516428 PMC: 1160320. DOI: 10.1113/jphysiol.1994.sp020024.

Inositol 1,4,5-trisphosphate receptor in skeletal muscle: differential expression in myofibres.

Moschella M, WATRAS J, Jayaraman T, Marks A J Muscle Res Cell Motil. 1995; 16(4):390-400.

PMID: 7499479 DOI: 10.1007/BF00114504.

Activation of inositol trisphosphate-sensitive Ca2+ channels of sarcoplasmic reticulum from frog skeletal muscle.

Suarez-Isla B, Alcayaga C, Marengo J, Bull R J Physiol. 1991; 441:575-91.

PMID: 1667801 PMC: 1180215. DOI: 10.1113/jphysiol.1991.sp018768.

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